Microarray Profiling and Functional Identification of LncRNA in Mice Intestinal Mucosa Following Intestinal Ischemia/Reperfusion
| Autor: | Yong Yang, Wei-Feng Liu, Ke-Xuan Liu, Qi-Wen Deng, Jian-Tong Shen, Wen-Jing Yang, Miao Xu |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Gene knockdown Microarray Biology Microarray Analysis Long non-coding RNA Cell biology Mice Inbred C57BL 03 medical and health sciences 0302 clinical medicine Intestinal mucosa ErbB 030220 oncology & carcinogenesis Reperfusion Injury Animals 030211 gastroenterology & hepatology Surgery RNA Long Noncoding RNA Messenger KEGG Intestinal Mucosa PI3K/AKT/mTOR pathway Reactive oxygen species metabolic process Cells Cultured |
| Zdroj: | The Journal of surgical research. 258 |
| ISSN: | 1095-8673 |
| Popis: | Background Intestinal ischemia-reperfusion (I/R) injury is a common clinical event with high mortality, but its mechanism is elusive. Although long noncoding RNAs (lncRNAs) have recently emerged as critical molecules in I/R damage in other organs, the changes in their expression and potential roles in intestinal I/R remain unclear. Methods The expression profiles of both lncRNAs and mRNAs in mouse intestinal mucosa after intestinal I/R were explored by a microarray approach, and their biological functions were elucidated by gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. Then, some lncRNAs were further verified by qRT-PCR. Based on the coding-noncoding gene coexpression (CNC) network analyses, the role of lncRNA AK089510 in intestinal I/R-induced intestinal mucosa apoptosis was investigated by knockdown assay in vitro. Results A total of 3602 aberrantly expressed lncRNAs (1503 upregulated and 2099 downregulated) and 3158 mRNAs (1528 upregulated and 1630 downregulated) were identified. The dysregulated transcripts were enriched in the lipid metabolic process, apoptotic process, reactive oxygen species metabolic process, MAPK, TNF, ErbB, mTOR, and FoxO signaling pathways, and so on. The overexpression of lncRNA AK089510 was validated by qRT-PCR, and the CNC analysis revealed its target mRNAs. AK089510-siRNA reduced Casp6 and Casp7 expression and suppressed intestinal epithelial cell apoptosis after oxygen-glucose deprivation treatment. Conclusions Our study revealed the lncRNA and mRNA expression patterns in mouse intestinal mucosa after intestinal I/R and predicted their potential functions and pathways. We identified AK089510 as a novel lncRNA involved in the apoptosis of intestinal mucosa, advancing our understanding of the molecular mechanisms of intestinal I/R injury. |
| Databáze: | OpenAIRE |
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