The Genetics of Differential Gene Expression Related to Fruit Traits in Strawberry (Fragaria ×ananassa)
| Autor: | Nadia Mourad, Sujeet Verma, Kevin M. Folta, Christopher R. Barbey, Anne E Schwartz, Vance M. Whitaker, Seonghee Lee, Max Hogshead |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
lcsh:QH426-470 Quantitative trait locus Biology Transcriptome 03 medical and health sciences transcriptomics 0302 clinical medicine Gene expression Genetics Gene Genetics (clinical) Original Research pectin strawberry (Fragaria ×ananassa Duch.) food and beverages Fragaria Phenotype anthocyanins pathway analysis lcsh:Genetics 030104 developmental biology Genetic marker eQTL analysis 030220 oncology & carcinogenesis Expression quantitative trait loci Molecular Medicine |
| Zdroj: | Frontiers in Genetics Frontiers in Genetics, Vol 10 (2020) |
| ISSN: | 1664-8021 |
| Popis: | Octoploid strawberry (Fragaria ×ananassa) is a major specialty crop under intense annual selection for traits relating to plant vigor and fruit quality. Most functional validation experiments rely on transgenic or transient gene expression assays in the mature receptacle. These findings are not typically translatable to breeding without identifying a natural genetic source of transcript level variation, and developing reliable markers for selection in octoploids. Expression QTL (eQTL) analysis is a genetic/transcriptomic association approach for identifying sequence variants predicting differential expression. This eQTL study analyzed a wide array of mature receptacle-expressed genes, encompassing the majority of total mature receptacle transcript accumulation and almost all strawberry genes described in the literature. These results identified segregating genetic variants associated with the differential expression of hundreds of strawberry genes, many with known interest to breeders. Several of these eQTL pertain to published genes whose expression levels have been demonstrated to influence mature receptacle phenotypes. Many include key genes of the phenylpropanoid pathway, vitamin C, carotenoid, pectin, and receptacle carbohydrate/sugar metabolism. These subgenome-specific genetic markers may allow breeders to select for desired ranges of target gene expression. These results may also guide basic research efforts and facilitate the identification of causal genes underlying trait QTL. |
| Databáze: | OpenAIRE |
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