The decline of atrial natriuretic peptide (ANP) gene expression in older rats and the effects of ginsenoside on ANP gene expression
| Autor: | Yan Jin, Arnold Boersma, Marie-Christine Vantyghem, Ming Hong, Yin-Qiao Mai, Jean Lefebvre, Kia-Ki Han |
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| Rok vydání: | 1992 |
| Předmět: |
Male
Aging medicine.medical_specialty Ginsenosides Physiology Dot blot Biology Biochemistry Ginseng chemistry.chemical_compound Atrial natriuretic peptide Internal medicine Gene expression medicine Animals RNA Messenger Northern blot Molecular Biology Rats Inbred Strains General Medicine Saponins Blotting Northern Rats Endocrinology Gene Expression Regulation chemistry Ageing Ginsenoside Circulatory system cardiovascular system Female DNA Probes Atrial Natriuretic Factor hormones hormone substitutes and hormone antagonists |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Comparative Biochemistry. 101:35-39 |
| ISSN: | 0305-0491 |
| Popis: | 1. 1. The levels of atrial natriuretic peptide(ANP) gene expression in rat atria at 2–3 and 14–18 months of age and the effects of ginsenosides on r-ANP gene expression by determining the concentration of ANP-mRNA were investigated. The male and female rats were abdominally (i.p.) injected with aqueous solution of ginsenosides prepared from ginseng stems and leaves (G-PSL) and ginseng roots (G-PR), 50 mg/kg body wt, once a day for 7 days. Atria total RNA was extracted by the cold phenol method. The ANP-mRNA contents were determined using the Northern blot and dot blot hybridization technique with α-32 ∗ P-labelled r-prepro-ANP-cDNA probe. 2. 2. The ANP-mRNA contents of 14–18 month rats were remarkably less than that of 2–3 month rats. The levels of male and female rats' atria at 14–18 months were about 15 and 60%, the content of male and female rats at 2–3 months respectively. 3. 3. G-PSL and G-PR increased the ANP-mRNA content of male rats at 14–18 months 1- and 2-fold, respectively, whereas G-PSL and G-PR decreased the ANP-mRNA content in male rats of age 2–3 months. 4. 4. These results revealed that the ANP gene expression declined during ontogenic ageing development and ginsenosides possessed anti-ageing effects in the heart endocrineous function aspect. |
| Databáze: | OpenAIRE |
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