Rotavirus VP6 protein mucosally delivered by cell wall-derived particles from Lactococcus lactis induces protection against infection in a murine model
| Autor: | Graciela Glikmann, Nicolás M. Gutierrez, Patricia Angelica Barril, Alejandro A. Castello, Marcelo G. Mandile, C. Facundo Temprana, Marcelo Horacio Argüelles, Laura Emilia Esteban, Dalila Silvestre |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
Rotavirus 0301 basic medicine Viral Diseases Physiology medicine.medical_treatment Protein Expression Biotecnología relacionada con la Salud lcsh:Medicine Pathology and Laboratory Medicine medicine.disease_cause Biochemistry Mice Immunologic Adjuvants Antibody Specificity Cell Wall Immune Physiology Lactococcus Medicine and Health Sciences Public and Occupational Health Enzyme-Linked Immunoassays purl.org/becyt/ford/3.4 [https] lcsh:Science Antigens Viral Drug Carriers Mice Inbred BALB C Vaccines Immune System Proteins Multidisciplinary Attenuated vaccine Vaccination and Immunization Recombinant Proteins Bacterial Pathogens Lactococcus lactis Infectious Diseases Medical Microbiology VP6 purl.org/becyt/ford/3 [https] Pathogens Antibody Adjuvant Research Article CIENCIAS MÉDICAS Y DE LA SALUD Infectious Disease Control Immunology Biology Research and Analysis Methods Microbiology Rotavirus Infections Antibodies Biotecnología de la Salud 03 medical and health sciences Immune system Gene Expression and Vector Techniques medicine Animals LACTOCOCCUS LACTIS Viral shedding Immunoassays Molecular Biology Techniques Microbial Pathogens Molecular Biology Rotavirus Infection Molecular Biology Assays and Analysis Techniques Mucous Membrane Bacteria lcsh:R Organisms Biology and Life Sciences Proteins biology.organism_classification Immunoglobulin A Disease Models Animal 030104 developmental biology Immunization Immunoglobulin G Immunologic Techniques biology.protein Capsid Proteins lcsh:Q Preventive Medicine ROTAVIRUS |
| Zdroj: | PLoS ONE, Vol 13, Iss 9, p e0203700 (2018) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET PLoS ONE |
| Popis: | Rotaviruses are the primary cause of acute gastroenteritis in children worldwide. Although the implementation of live attenuated vaccines has reduced the number of rotavirus-associated deaths, variance in their effectiveness has been reported in different countries. This fact, among other concerns, leads to continuous efforts for the development of new generation of vaccines against rotavirus.In this work, we describe the obtention of cell wall-derived particles from a recombinant Lactococcus lactis expressing a cell wall-anchored version of the rotavirus VP6 protein. After confirming by SDS-PAGE, Western blot, flow cytometry and electronic immunomicroscopy that these particles were carrying the VP6 protein, their immunogenic potential was evaluated in adult BALB/c mice. For that, mucosal immunizations (oral or intranasal), with or without the dmLT [(double mutant Escherichia coli heat labile toxin LT(R192G/L211A)] adjuvant were performed. The results showed that these cell wall-derived particles were able to generate anti-rotavirus IgG and IgA antibodies only when administered intranasally, whether the adjuvant was present or not. However, the presence of dmLT was necessary to confer protection against rotavirus infection, which was evidenced by a 79.5 percent viral shedding reduction.In summary, this work describes the production of cell wall-derived particles which were able to induce a protective immune response after intranasal immunization. Further studies are needed to characterize the immune response elicited by these particles as well as to determine their potential as an alternative to the use of live L. lactis for mucosal antigen delivery. Fil: Temprana, Carlos Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina Fil: Argüelles, Marcelo Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina Fil: Gutierrez, Nicolás Manuel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina Fil: Barril, Patricia Angelica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigación y Asistencia Técnica a la Industria; Argentina Fil: Esteban, Laura Emilia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Silvestre, Dalila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina Fil: Mandile, Marcelo Gastón. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Almallo de Glikmann, Graciela. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina Fil: Castello, Alejandro Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Virologia; Argentina. Universidad Nacional Arturo Jauretche; Argentina |
| Databáze: | OpenAIRE |
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