Radiation sensitivity of human B-lineage lymphoid precursor cells
| Autor: | Fatih M. Uckun, Vedat Obuz, Kevin G. Waddick, Chae Heonjoo Park, Lahcen Oubaha, Chang W. Song, Woo Sung Min, Norman Friedman, James Mitchell |
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| Rok vydání: | 1991 |
| Předmět: |
Cancer Research
Genotype Proliferation index Cell Survival Intracellular pH Radiation Tolerance Immunophenotyping Radiation sensitivity Precursor cell Tumor Cells Cultured Humans Medicine Radiology Nuclear Medicine and imaging Radiosensitivity Receptor Neoplasm Staging B-Lymphocytes Radiation business.industry Precursor Cell Lymphoblastic Leukemia-Lymphoma Molecular biology Oncology Cell culture Immunology Immunoglobulin heavy chain lipids (amino acids peptides and proteins) business |
| Zdroj: | International Journal of Radiation Oncology*Biology*Physics. 21:1553-1560 |
| ISSN: | 0360-3016 |
| Popis: | We studied the radiation sensitivity of eight immunophenotypically distinct B-lineage lymphoid precursor cell (LPC) lines of acute lymphoblastic leukemia (ALL) or fetal liver origin corresponding to discrete developmental stages of human B-cell ontogeny. The radiation sensitivity of B-lineage LPC showed a temporal association with the distinct stages of development. FL112 and FL114 fetal liver pro-B cells (Stage 0 B-lineage LPC) with germline immunoglobulin heavy chain (IgH) genes but rearranged T-cell receptor gamma (T γ ) genes (D O of FL112 = 80.3 cGy, D O of FL114 = 50.2 cGy), REH ALL pre-pre-B cells (Stage I B-lineage LPC) with rearranged IgH and T γ genes (D O = 66.1 cGy), and NALM-6 ALL pre-pre-B/pre-B cells (Stage II B-lineage LPC) (D O = 50.5 cGy) corresponding to the earliest three stages of human B-lymphocyte development were the most radiation sensitive B-lineage LPC populations. By comparison, KM-3 ALL pre-B (Stage III B-lineage LPC) (D O = 194.7 cGy), HPB-NULL ALL pre-B (Stage IV B-lineage LPC) (D O = 134.6 cGy), and sIgM + RAJI/NAMALWA early B (Stage Va/b B-lineage LPC) cell lines (D O of RAJI = 144.0 cGy, D O of NAMALWA = 165.5 cGy) corresponding to the later stages of human B-lymphocyte development were much more radiation resistant. These results indicate that the radiation sensitivity of B-lineage LPC decreases during maturation within the B-lineage lymphoid precursor pathway. By comparison, the S-phase index (% of S-phase cells as determined by DNA flow cytometry) or proliferation index (% S+G 2 M), cellular protein content, intracellular glutathione (GSH) level, glutathione-S-transferase (GST) activity, intracellular pH, or free cytoplasmic calcium concentration did not correlate with the radiation sensitivity of the B-lineage LPC. |
| Databáze: | OpenAIRE |
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