Effect of dietary polyphenols on K562 leukemia cells: a Foodomics approach
Autor: | Alberto Valdés, Clara Ibáñez, Lourdes Rocamora-Reverte, Carolina Simó, Virginia García-Cañas, Alejandro Cifuentes, José A. Ferragut |
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Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Ministerio de Educación y Ciencia (España) |
Rok vydání: | 2012 |
Předmět: |
Cell Survival
Clinical Biochemistry Foodomics Biology Bioinformatics Biochemistry Polymerase Chain Reaction Analytical Chemistry Transcriptome Metabolomics medicine Metabolome Humans Transcriptomics Plant Extracts Gene Expression Profiling Cell Cycle Electrophoresis Capillary Polyphenols medicine.disease Rosmarinus Gene expression profiling Gene Expression Regulation Neoplastic Leukemia Metabolic pathway Dietary polyphenols Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Leukemia cells K562 Cells K562 cells |
Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
ISSN: | 1522-2683 |
Popis: | In this work, a global Foodomics strategy has been applied to study the antiproliferative effect of dietary polyphenols from rosemary on two human leukemia lines, one showing a drug-sensitive phenotype (K562), and another exhibiting a drug-resistant phenotype (K562/R). To this aim, whole-transcriptome microarray together with an MS-based nontargeted analytical approach (via CE-TOF MS and UPLC-TOF MS) have been employed to carry out transcriptomics and metabolomics analyses, respectively. Functional enrichment analysis was done using ingenuity pathway analysis (IPA) software as a previous step for a reliable interpretation of transcriptomic and metabolomic profiles. Rosemary polyphenols altered the expression of approximately 1% of the genes covered by the whole transcriptome microarray in both leukemia cell lines. Overall, differences in the transcriptional induction of a number of genes encoding phase II detoxifying and antioxidant genes, as well as differences in the metabolic profiles observed in the two leukemia cell lines suggest that rosemary polyphenols may exert a differential chemopreventive effect in leukemia cells with different phenotypes. IPA predictions on transcription factor analysis highlighted inhibition of Myc transcription factor function by rosemary polyphenols, which may explain the observed antiproliferative effect of rosemary extract in the leukemia cells. Metabolomics analysis suggested that rosemary polyphenols affected differently the intracellular levels of some metabolites in two leukemia cell sublines. Integration of data obtained from transcriptomics and metabolomics platforms was attempted by overlaying datasets on canonical (defined) metabolic pathways using IPA software. This strategy enabled the identification of several differentially expressed genes in the metabolic pathways modulated by rosemary polyphenols providing more evidences on the effect of these compounds. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Funded by: Ministerio de Ciencia e Innovación, Spain. Grant Numbers: AGL2008-05108-C03-01, AGL2008-05108-C03-02, AGL2011-29857-C03-01 and Ministerio de Educacion y Ciencia, Spain. Grant Number: CSD2007-00063 FUN-C-FOOD. |
Databáze: | OpenAIRE |
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