Effects of nitric oxide system and osmotic stress on Aquaporin-1 in the postnatal heart

Autor: Vanina Netti, Mariana C. Vatrella, Agustina N. Iovane, Andrea L. Fellet, Elsa Zotta, Ana M. Balaszczuk
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Male
Cell Membrane Permeability
Blood Pressure
030204 cardiovascular system & hematology
Rats
Sprague-Dawley
chemistry.chemical_compound
0302 clinical medicine
Scattering
Radiation
Dehydration
Heart
purl.org/becyt/ford/3.1 [https]
General Medicine
Immunohistochemistry
Postnatal Growth
Medicina Básica
NG-Nitroarginine Methyl Ester
Biochemistry
Aquaporin 1
purl.org/becyt/ford/3 [https]
Sodium nitroprusside
medicine.drug
medicine.medical_specialty
CIENCIAS MÉDICAS Y DE LA SALUD
Osmotic shock
Systole
Heart Ventricles
Nitrosation
Biology
Nitric Oxide
Fisiología
Models
Biological
Nitric oxide
03 medical and health sciences
Osmotic Pressure
Internal medicine
medicine
Animals
Nitric Oxide Donors
Pharmacology
Water transport
Sarcolemma
Membranes
Myocardium
Cell Membrane
Colocalization
Reproducibility of Results
Water
Aquaporin-1
030104 developmental biology
Endocrinology
chemistry
Animals
Newborn
Nitric Oxide Synthase
Homeostasis
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
Popis: Aquaporin-1 (AQP1) is expressed in the heart and its relationship with NO system has not been fully explored. The aims of this work were to study the effects of NO system inhibition on AQP1 abundance and localization and evaluate AQP1 S-nitrosylation in a model of water restriction during postnatal growth. Rats aged 25 and 50 days (n = 15) were divided in: R: water restriction; C: water ad libitum; RL: L-NAME (4 mg/kg day) + water restriction; CL: L-NAME + water ad libitum. AQP1 protein levels, immunohistochemistry and S-nitrosylation (colocalization of AQP1 and S-nitrosylated cysteines by confocal microscopy) were determined in cardiac tissue. We also evaluated the effects of NO donor sodium nitroprusside (SNP) on osmotic water permeability of cardiac membrane vesicles by stopped-flow spectrometry. AQP1 was present in cardiac vascular endothelium and endocardium in C and CL animals of both ages. Cardiac AQP1 levels were increased in R50 and RL50 and appeared in cardiomyocyte plasma membrane. No changes in AQP1 abundance or localization were observed in R25, but RL25 group showed AQP1 presence on cardiomyocyte sarcolemma. AQP1 S-nitrosylation was increased in R25 group, without changes in the 50-day-old group. Cardiac membrane vesicles expressing AQP1 presented a high water permeability coefficient and pretreatment with SNP decreased water transport. Age-related influence of NO system on AQP1 abundance and localization in the heart may affect cardiac water homeostasis during hypovolemic state. Increased AQP1 S-nitrosylation in the youngest group may decrease osmotic water permeability of cardiac membranes, having a negative impact on cardiac water balance. Fil: Netti, Vanina Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiología Humana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Iovane, Agustina N.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiología Humana; Argentina Fil: Vatrella, Mariana C.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiología Humana; Argentina Fil: Zotta, Elsa. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina Fil: Fellet, Andrea L.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiología Humana; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Balaszczuk, Ana Maria. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiología Humana; Argentina
Databáze: OpenAIRE
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