In situ evaluation of the toxicological impact of a wastewater effluent on the fish Prochilodus lineatus: biochemical and histological assessment
Autor: | Jimena Cazenave, Carla Bacchetta, María Rita Pérez, Andrea Silvana Rossi, Pedro Carriquiriborde, Yanina Elorriaga |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Gill BIOMARKER General Decision Sciences Sewage 010501 environmental sciences medicine.disease_cause 01 natural sciences CASPASE 3 Transaminase Toxicology Ciencias Biológicas purl.org/becyt/ford/1 [https] 03 medical and health sciences medicine Food science OXIDATIVE STRESS purl.org/becyt/ford/1.6 [https] Effluent Ecology Evolution Behavior and Systematics 0105 earth and related environmental sciences Pollutant Ecology biology business.industry Biología Marina Limnología biology.organism_classification 030104 developmental biology Wastewater Prochilodus lineatus CAGING EXPERIMENT business Oxidative stress CIENCIAS NATURALES Y EXACTAS |
Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
Popis: | Sewage effluents are the most important source of emergent pollutants in the aquatic environment. In thepresent study the toxicological impact of an untreated sewage effluent on the Prochilodus lineatus fish was assessedunder field conditions using a caging experiment. The biomarkers which were measured here involvedoxidative stress markers, hepatic function parameters, neurotoxicity indicators, energy reserves, histologicalalterations and brain cell proliferation. In addition, water quality parameters including the occurrence of somehuman pharmaceuticals were measured. Juveniles of P. lineatus were caged for 15 days at three sites: the effluentsite, and 2 km upstream and downstream from the effluent discharge. Caffeine, atenolol, carbamazepine, enalapriland sildenafil were detected in water river samples. The increased activation of caspase-3 and the decreasedcell proliferation in the diencephalon showed that the brain of fish caged at the effluent was affected.These fish also displayed a rise in hepatic transaminase activity, and oxidative stress in liver and gills which wasevidenced by an increased lipid peroxidation and activation of antioxidant enzymes. At tissue level, increasedglycogen and decreased lipid contents in liver as well as the highest indexes of hepatic lesions were also observedin the fish caged at the effluent. Both biochemical and histopathological findings demonstrated that effects weremore severe on the liver of such fish than on their gills. The in situ exposure method carried out in our studymakes it possible to observe the real effects of the sewage effluent on fish. Furthermore, our results also provide abetter understanding of the harmful effects of wastewater effluents on the aquatic wildlife. Fil: Pérez, María Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Rossi, Andrea Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Bacchetta, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Elorriaga, Yanina. Universidad Nacional de La Plata; Argentina Fil: Carriquiriborde, Pedro. Universidad Nacional de La Plata; Argentina Fil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina |
Databáze: | OpenAIRE |
Externí odkaz: |