S-(−)-10,11-Dihydroxyfarnesoic Acid Methyl Ester Inhibits Melanin Synthesis in Murine Melanocyte Cells
| Autor: | Sang-Han Lee, Seung-Hwa Baek, Cheol-Sik Yoon, Jun-Won Ahn, Jae-Cheon Shin, Sung-Hee Nam |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Tyrosinase
insect juvenile hormone tyrosinase Biology Melanocyte Article Catalysis Cell Line lcsh:Chemistry Inorganic Chemistry Melanin Mice whitening medicine Animals Zymography RNA Messenger Beauveria Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy Melanins Microphthalmia-Associated Transcription Factor integumentary system Monophenol Monooxygenase Organic Chemistry General Medicine Microphthalmia-associated transcription factor melanin Computer Science Applications Intramolecular Oxidoreductases Juvenile Hormones inhibitor dihydroxyfarnesoic acid medicine.anatomical_structure lcsh:Biology (General) lcsh:QD1-999 Biochemistry Cell culture Juvenile hormone Melanocytes insect juvenilehormone Oxidoreductases Intracellular |
| Zdroj: | International Journal of Molecular Sciences Volume 15 Issue 7 Pages 12750-12763 International Journal of Molecular Sciences, Vol 15, Iss 7, Pp 12750-12763 (2014) INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(15): 7 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms150712750 |
| Popis: | The development of antimelanogenic agents is important for the prevention of serious aesthetic problems such as melasmas, freckles, age spots, and chloasmas. In the course of screening for melanin synthesis inhibitors, we found that the culture broth from an insect morphopathogenic fungus, Beauveria bassiana CS1029, exhibits potent antimelanogenic activity. We isolated and purified an active metabolite and identified it as S-(-)-10,11-dihydroxyfarnesoic acid methyl ester (dhFAME), an insect juvenile hormone. To address whether dhFAME inhibits melanin synthesis, we first measured the size of the melanin biosynthesis inhibition zone caused by dhFAME. dhFAME also showed inhibitory activity against mushroom tyrosinase in Melan-a cells. Intracellular, dose-dependent tyrosinase inhibition activity was also confirmed by zymography. In addition, we showed that dhFAME strongly inhibits melanin synthesis in Melan-a cells. Furthermore, we compared levels of TYR, TRP-1, TRP-2, MITF, and MC1R mRNA expression by reverse-transcription polymerase chain reaction and showed that treatment of Melan-a cells with 35 mu M dhFAME led to an 11-fold decrease in TYR expression, a 6-fold decrease in TRP-2 expression, and a 5-fold decrease in MITF expression. Together, these results indicate that dhFAME is a potent inhibitor of melanin synthesis that can potentially be used for cosmetic biomaterial(s). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|