Gas chromatography-mass spectrometric assay for propofol in cerebrospinal fluid of traumatic brain patients
| Autor: | Donald R. A. Uges, Joukje van der Naalt, Hiltjo Kuiper, Ben Greijdanus, Mariska Y. M. Peeters, Catherijne A. J. Knibbe |
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| Přispěvatelé: | Faculteit Medische Wetenschappen/UMCG, Groningen Research Institute of Pharmacy, Molecular Neuroscience and Ageing Research (MOLAR) |
| Rok vydání: | 2006 |
| Předmět: |
Adult
Male EXTRACTION BLOOD QUANTITATION Adolescent SAMPLES Traumatic brain injury Clinical Biochemistry CSF Biochemistry High-performance liquid chromatography Sensitivity and Specificity Gas Chromatography-Mass Spectrometry Analytical Chemistry law.invention Cerebrospinal fluid law medicine Humans HUMAN PLASMA Propofol Intracranial pressure Detection limit ANESTHESIA Chromatography Chemistry INFUSION Reproducibility of Results Cell Biology General Medicine medicine.disease Intensive care unit Brain Injuries GC-MS HPLC Gas chromatography–mass spectrometry Anesthetics Intravenous medicine.drug |
| Zdroj: | Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 852(1-2), 635-639. ELSEVIER SCIENCE BV |
| ISSN: | 1570-0232 |
| Popis: | A sensitive gas chromatography-mass spectrometry method for measuring propofol in cerebrospinal fluid is described, validated and applied to four patients after traumatic brain injury. The limit of quantitation was 2 mu g/L using a volume of 0.5 mL. The inter- and intra-assay coefficients of variation were 5.9 and 5.1%, respectively. The assay covers the linear concentration range of 2-50 mu g/L, which is relevant in clinical pharmacokinetic studies when propofol is used in the Intensive Care Unit as a sedative agent or to lower the intracranial pressure. (c) 2007 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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