On-line coupling of solid-phase extraction and capillary electrophoresis for the determination of cefoperazone and ceftiofur in plasma
| Autor: | Gerhardus J. de Jong, Carme Aguilar, Marta Calull, F. W. Alexander Tempels, Francesc Borrull, Patricia Puig, Govert W. Somsen |
|---|---|
| Přispěvatelé: | BioAnalytical Chemistry, AIMMS |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Clinical Biochemistry
capillary electrophoresis Cefoperazone Biochemistry Analytical Chemistry Capillary electrophoresis medicine Animals Humans solid-phase extraction plasma samples Solid phase extraction Antibacterial agent Detection limit Chromatography Elution Chemistry Extraction (chemistry) Electrophoresis Capillary Reproducibility of Results Cell Biology General Medicine Anti-Bacterial Agents cephalosporins Cattle Spectrophotometry Ultraviolet Ceftiofur on-line medicine.drug |
| Zdroj: | Puig, P, Tempels, F W A, Borrull, F, Calull, M, Aguilar, C, Somsen, G W & de Jong, G J 2007, ' coupling of solid-phase extraction and capillary electrophoresis for the determination of cefoperazone and ceftiofur in plasma ', Journal of Chromatography B, vol. 856, no. 1-2, pp. 365-370 . https://doi.org/10.1016/j.jchromb.2007.05.030 Journal of Chromatography B, 856(1-2), 365-370. Elsevier |
| ISSN: | 1873-376X 1570-0232 |
| DOI: | 10.1016/j.jchromb.2007.05.030 |
| Popis: | We present a method for determining two cephalosporins (cefoperazone and ceftiofur) in plasma by on-line solid-phase extraction (SPE)-capillary zone electrophoresis (CZE) with a T-split interface. Using this interface, a part of the SPE elution plug containing the cephalosporins is injected while the rest of the sample is flushed to waste. SPE was carried out using a C(18) micro-precolumn and the cephalosporins presented good retention properties with breakthrough volumes above 1 ml. Using a desorption volume of 426 nl of acetonitrile, recoveries were 75 and 90%, for cefoperazone and ceftiofur, respectively. The resulting elution volume was about 1.8 microl. A deproteinization step was included prior to SPE for the analysis of plasma samples with recoveries of 90 and 57% for cefoperazone and ceftiofur, respectively. With UV detection at 254 nm, linear relationships between the injected concentration and peak area was measured between 10 and 500 ng ml(-1) for standards, and 200 and 1500 ng ml(-1) for plasma samples. Intra-day (n=5) and inter-day (n=5) peak area repeatability were lower than 12% RSD. The detection limits obtained for spiked plasma (100 ng ml(-1) cefoperazone and ceftiofur) are sufficient for applying the method to pharmacokinetic studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect