Small Rho GTPase Rac1 determines human epidermal stem cell fate in vitro
| Autor: | Rui Guo, Linlin Chai, Lu Gan, Xia Dai, Chuan Cao, Sheng Bi, Shirong Li |
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| Rok vydání: | 2010 |
| Předmět: |
Keratinocytes
rac1 GTP-Binding Protein Cellular differentiation Recombinant Fusion Proteins Cell RAC1 Biology Genetics medicine Cell Adhesion Humans Cell adhesion Child reproductive and urinary physiology Tissue homeostasis Cells Cultured Cell Proliferation urogenital system Cell growth Stem Cells Cell Cycle Cell Differentiation General Medicine Cell cycle Cell biology medicine.anatomical_structure Epidermal Cells embryonic structures Cancer research biological phenomena cell phenomena and immunity Stem cell |
| Zdroj: | International journal of molecular medicine. 25(5) |
| ISSN: | 1791-244X |
| Popis: | Epidermal stem cells (ESCs) are essential not only for tissue homeostasis but also for skin to respond to insults, but the mechanisms of stem cell regulation are unknown. To investigate the function of Rac1 in ESC development, we introduced either the dominant negative isoform or constitutively the active mutant of Rac1 in cultured human ESCs by using a retroviral vector and then analyzed the consequences. Upon activation, Rac1 increased surface alpha6/beta1 integrin levels and promoted colony forming efficiency of ESCs. Conversely, dominant negative Rac1 caused a progressive reduction in growth rate, an inhibition of adhesiveness and a marked stimulation of terminal differentiation, without any effect on the cell cycle. These results were consistent with the role of Rac1 in determining the fate of ESCs by controlling their exit from the stem cell compartment. Our results reveal a novel biological role for Rac1 and provide new insights into the mechanism regulating ESCs. |
| Databáze: | OpenAIRE |
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