Adult rat bone marrow stromal cells express genes associated with dopamine neurons
| Autor: | Ira B. Black, Brian C. Kramer, Dale Woodbury |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Patched Receptors
medicine.medical_specialty Receptor complex Glial Cell Line-Derived Neurotrophic Factor Receptors Stromal cell Dopamine Kruppel-Like Transcription Factors Biophysics Bone Marrow Cells Receptors Cell Surface Zinc Finger Protein GLI1 Biochemistry Receptors G-Protein-Coupled Growth factor receptor Internal medicine Nuclear Receptor Subfamily 4 Group A Member 2 Glial cell line-derived neurotrophic factor medicine Animals Molecular Biology Cells Cultured Neurons Otx Transcription Factors biology Reverse Transcriptase Polymerase Chain Reaction Dopaminergic Mesenchymal stem cell Cell Biology Smoothened Receptor Rats Cell biology DNA-Binding Proteins Endocrinology Nuclear receptor Fibroblast growth factor receptor biology.protein Stromal Cells Transcription Factors |
| Zdroj: | Biochemical and Biophysical Research Communications. 343:1045-1052 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2006.02.191 |
| Popis: | An intensive search is underway to identify candidates to replace the cells that degenerate in Parkinson's disease (PD). To date, no suitable substitute has been found. We have recently found that adult rat bone marrow stromal cells (MSCs) can be induced to assume a neuronal phenotype in vitro. These findings may have particular relevance to the treatment of PD. We now report that adult MSCs express multiple dopaminergic genes, suggesting that they are potential candidates for cell therapy. Using RT-PCR, we have examined families of genes that are associated with the development and/or survival of dopaminergic neurons. MSCs transcribe a variety of dopaminergic genes including patched and smoothened (components of the Shh receptor), Gli-1 (downstream mediator of Shh), and Otx-1, a gene associated with formation of the mesencephalon during development. Furthermore, Shh treatment elicits a 1.5-fold increase in DNA synthesis in cultured MSCs, suggesting the presence of a functional Shh receptor complex. We have also found that MSCs transcribe and translate Nurr-1, a nuclear receptor essential for the development of dopamine neurons. In addition, MSCs express a variety of growth factor receptors including the glycosyl-phosphatidylinositol-anchored ligand-binding subunit of the GDNF receptor, GFRalpha1, as well as fibroblast growth factor receptors one and four. The expression of genes that are associated with the development and survival of dopamine neurons suggests a potential role for these cells in the treatment of Parkinson's disease. |
| Databáze: | OpenAIRE |
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