Brain-Derived Neurotrophic Factor Modulates Intestinal Barrier by Inhibiting Intestinal Epithelial Cells Apoptosis in Mice
| Autor: | X L Zuo, X Li, D Y Zhao, Wenxue Zhang, Y B Yu, Q Q Qi, X Long |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Physiology Apoptosis Caspase 3 Occludin Mice 03 medical and health sciences 0302 clinical medicine Intestinal mucosa Animals Intestinal Mucosa Barrier function Mice Knockout Brain-derived neurotrophic factor Tight Junction Proteins Tight junction Ussing chamber Chemistry Brain-Derived Neurotrophic Factor Epithelial Cells General Medicine Molecular biology Mice Inbred C57BL 030104 developmental biology Female 030211 gastroenterology & hepatology |
| Zdroj: | Physiological Research. :475-485 |
| ISSN: | 1802-9973 0862-8408 |
| DOI: | 10.33549/physiolres.933641 |
| Popis: | We aimed to investigate the effects of brain-derived neurotrophic factor (BDNF) on apoptosis of intestinal epithelial cells (IECs) and alterations of intestinal barrier integrity using BDNF knock-out mice model. Colonic tissues from BDNF+/+ mice and BDNF+/- mice were prepared for this study. The integrity of colonic mucosa was evaluated by measuring trans-mucosa electrical resistance and tissue conductance in Ussing chamber. The colonic epithelial structure was analyzed by transmission electron microscopy. Apoptosis involvement was determined with TUNEL staining, active caspase-3 immunostaining and Western blotting for the protein expression of active caspase-3, Bax and Bcl-2. The expression levels and distribution of tight junction proteins were evaluated by immunohistochemistry or Western blots. Compared with BDNF+/+ mice, BDNF+/- mice displayed impaired integrity and ultrastructure alterations in their colonic mucosa, which was characterized by diminished microvilli, mitochondrial swelling and epithelial cells apoptosis. Altered intestinal barrier function was linked to excessive apoptosis of IECs demonstrated by the higher proportion of TUNEL-positive apoptotic cells and enhanced caspase activities in BDNF+/- mice. Increased expression of Bax and claudin-2 proteins and reduced Bcl-2 and tight junction proteins (occludin, ZO-1 and claudin-1) expression were also detected in the colonic mucosa of BDNF+/- mice. BDNF may play a role in the maintenance of intestinal barrier integrity via its anti-apoptotic properties. |
| Databáze: | OpenAIRE |
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