Correction and reporting of potassium results in haemolysed samples
| Autor: | Alan E Clague, Peter E. Hickman, Goce Dimeski |
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| Rok vydání: | 2005 |
| Předmět: |
Analyte
Pathology medicine.medical_specialty Hyperkalemia Potassium Clinical Biochemistry chemistry.chemical_element Hemolysis Specimen Handling medicine Humans False Positive Reactions Blood Specimen Collection Chromatography business.industry General Medicine Haemolysis medicine.disease Laboratory test chemistry medicine.symptom business Observer variation Mathematics |
| Zdroj: | Annals of Clinical Biochemistry: International Journal of Laboratory Medicine. 42:119-123 |
| ISSN: | 1758-1001 0004-5632 |
| DOI: | 10.1258/0004563053492739 |
| Popis: | Background: Potassium is usually the most important analyte affected by in vitro haemolysis and the result obtained may falsely indicate or disguise a life-threatening abnormality and so give rise to inappropriate treatment. The purpose of the study was to provide a solution to the problem of reporting potassium on haemolysed samples, taking into account both clinical needs and analytical concerns (inter-individual and inter-sample variability). Methods: Using a new procedure that mimics the collection process in an actual clinical setting, haemolysed samples were prepared from 41 volunteers with a range of inter-individual factors - haemoglobin 80-173 g/L, red blood cells 2.42-6.77 x 1012/L, leucocytes 3.0-306 x 109 /L and platelets 31-710 x 109/L - in order to develop a more accurate correction equation using a haemolytic index (HI) corresponding to g Hb/L in plasma. Results: The mean (range) potassium increase was 0.0036 mmol/L (0.0029-0.0053 mmol/L) per unit HI. The following equation was developed to estimate potassium increase per HI, in order to compensate approximately for potassium leakage in haemolysed samples: Corrected K+=Measured K+ -(HI x 0.004). Conclusion: The balanced solution is this: instead of reporting the post-haemolysis corrected potassium result a qualitative comment is given, indicating the likely range of the potassium concentration. If the potassium result is in a critically low or high range, it is communicated promptly to the requesting clinician. |
| Databáze: | OpenAIRE |
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