Amino acid residues Arg(659), Arg(660), and Tyr(661) in the spacer domain of ADAMTS13 are critical for cleavage of von Willebrand factor
| Autor: | X. Long Zheng, Sheng-Yu Jin, Christopher G. Skipwith |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Von Willebrand factor type C domain
Models Molecular Von Willebrand factor type A domain Recombinant Fusion Proteins Immunology Molecular Sequence Data ADAMTS13 Protein Arginine Biochemistry Protein Structure Secondary Thrombosis and Hemostasis Structure-Activity Relationship Protein structure Von Willebrand factor hemic and lymphatic diseases von Willebrand Factor Humans Amino Acid Sequence Peptide sequence Cells Cultured chemistry.chemical_classification biology Chemistry ADAMTS Cell Biology Hematology Molecular biology ADAMTS13 Amino acid Protein Structure Tertiary ADAM Proteins Kinetics Immobilized Proteins biology.protein Tyrosine Mutant Proteins Stress Mechanical Protein Multimerization Peptides Rheology Protein Binding |
| Zdroj: | Blood. 115(11) |
| ISSN: | 1528-0020 |
| Popis: | Previous studies have shown that ADAMTS13 spacer domain is required for cleavage of von Willebrand factor (VWF). However, the exact amino acid residues within this domain critical for substrate recognition are not known. Epitope mapping of anti-ADAMTS13 immunoglobulin G from patients with thrombotic thrombocytopenic purpura and sequence alignment of the ADAMTS13 spacer domains of human, mouse, and zebrafish with these of human and murine ADAMTS1, a closely related member of ADAMTS family, have provided hints to investigate the role of the amino acid residues between Arg659 and Glu664 of the ADAMTS13 spacer domain in substrate recognition. A deletion of all these 6 amino acid residues (ie, Arg659-Glu664) from the ADAMTS13 spacer domain resulted in dramatically reduced proteolytic activity toward VWF73 peptides, guanidine-HCl denatured VWF, and native VWF under fluid shear stress, as well as ultralarge VWF on endothelial cells. Site-directed mutagenesis, kinetic analyses, and peptide inhibition assays have further identified a role for amino acid residues Arg659, Arg660, and Tyr661 in proteolytic cleavage of various substrates under static and fluid shear stress conditions. These findings may provide novel insight into the structural-function relationship of ADAMTS13 and help us to understand pathogenesis of thrombotic thrombocytopenic purpura and other arterial thromboses associated with compromised VWF proteolysis. |
| Databáze: | OpenAIRE |
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