Prevalence of Aminoglycoside-Modifying Enzymes Genes Among Isolates ofEnterococcus faecalisandEnterococcus faeciumin Iran
| Autor: | Leila Shokrzadeh, Sara Sayadi, Ahmad Asgharzadeh, Mohammad Mehdi Feizabadi, Parviz Maleknejad, Sorror Asadi |
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| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
Enterococcus faecium Immunology Microbial Sensitivity Tests Iran Microbiology Enterococcus faecalis Minimum inhibitory concentration Drug Resistance Bacterial Prevalence medicine Tobramycin Humans Gram-Positive Bacterial Infections Pharmacology biology Kanamycin biochemical phenomena metabolism and nutrition biology.organism_classification Anti-Bacterial Agents Aminoglycosides Amikacin Gentamicin Netilmicin medicine.drug |
| Zdroj: | Microbial Drug Resistance. 12:265-268 |
| ISSN: | 1931-8448 1076-6294 |
| Popis: | Disks containing 120 microg of gentamicin were used to detect high-level gentamicin-resistant phenotype (HLGR) among isolates of Enterococcus faecalis (n = 79) and E. faecium (n = 35). These isolates were collected from three hospitals in Tehran during 2002-2004. The macrobroth dilution assay was then used to determine the minimum inhibitory concentration (MIC) of gentamicin. The susceptibility of isolates against amikacin, netilmicin, tobramycin, and kanamycin were also determined by Kirby-Bauer method. All isolates were subjected to polymerase chain reaction (PCR) assays targeting aminoglycoside modifying enzyme (AMEs) genes including aac(6 ')-aph(2 "), aph(2 ")-Ib, aph(2 ")-Ic, aph(2 ")-Ia, aph(2 ")-Id, aph(3 ')-IIIa, and ant(4 ')-Ia. Fifty-nine isolates (52%) showed HLGR phenotype. All isolates with HLGR phenotype and those showing 64MIC500 microg/ml contained aac(6 ')-aph(2 "). The aph(3 ')-IIIa was found in 61% of the isolates with HLGR phenotypes and in 65% of isolates with MIC500. Coexistence of aac(6 ')-aph(2 ") and aph(3 ')-IIIa gene among HLGR isolates of E. faecalis and E. faecium were 60% and 65%, respectively. The gene aph(2 ")-Ic was amplified in two isolates of E. faecium. The results of PCR for aph(2 ")-Id, ant(4 ')-Ia and aph(2 ")-Ib genes were negative. The aac(6 ')-aph(2 ") was the most frequent gene encoding resistance to gentamicin and other aminoglycosides followed by aph(3 ')-IIIa. Isolates lacking these genes were susceptible to all aminoglyocosides tested in this study. |
| Databáze: | OpenAIRE |
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