TIM-3 inhibits PDGF-BB-induced atherogenic responses in human artery vascular smooth muscle cells

Autor: Rui-Ming Liu, Junbing Lv, Baohong Jiang, He Rongzhou, Wen Li, Chong Lian, Jinsong Wang, Jiacong Qiu, Shenming Wang, Zhecun Wang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Cancer Research
Vascular smooth muscle
Becaplermin
migration
Biochemistry
Muscle
Smooth
Vascular
0302 clinical medicine
Cell Movement
Hepatitis A Virus Cellular Receptor 2
biology
Chemistry
NF-kappa B
Articles
Arteries
Arteriosclerosis Obliterans
Middle Aged
medicine.anatomical_structure
Oncology
Lower Extremity
030220 oncology & carcinogenesis
Molecular Medicine
Tumor necrosis factor alpha
Female
medicine.symptom
Platelet-derived growth factor receptor
Artery
medicine.medical_specialty
proliferation
Protein Array Analysis
Inflammation
T-cell immunoglobulin and mucin domain 3
Cell Line
03 medical and health sciences
Internal medicine
platelet-derived growth factor-BB
Genetics
medicine
human artery vascular smooth muscle cells
Humans
Molecular Biology
Aged
Cell Proliferation
Oncogene
Interleukin-6
Tumor Necrosis Factor-alpha
Atherosclerosis
Molecular medicine
030104 developmental biology
Endocrinology
Apoptosis
inflammation
biology.protein
Transcriptome
Zdroj: Molecular Medicine Reports
ISSN: 1791-3004
1791-2997
Popis: Increasing evidence suggests that T‑cell immunoglobulin and mucin domain 3 (TIM‑3) displays anti‑atherosclerotic effects, but its role in vascular smooth muscle cells (VSMCs) has not been reported. The present study aimed to investigate the function of TIM‑3 and its roles in human artery VSMCs (HASMCs). A protein array was used to investigate the TIM‑3 protein expression profile, which indicated that TIM‑3 expression was increased in the serum of patients with lower extremity arteriosclerosis obliterans disease (LEAOD) compared with healthy individuals. Immunohistochemistry and western blotting of arterial tissue further revealed that TIM‑3 expression was increased in LEAOD artery tissue compared with normal artery tissue. Additionally, platelet‑derived growth factor‑BB (PDGF‑BB) displayed a positive correlation with TIM‑3 expression in HASMCs. TIM‑3 decreased the migration and proliferation of PDGF‑BB‑induced HASMCs, and anti‑TIM‑3 blocked the effects of TIM‑3. The effect of TIM‑3 on the proliferation and migration of HASMCs was further investigated using LV‑TIM‑3‑transduced cells. The results revealed that TIM‑3 also inhibited PDGF‑BB‑induced expression of the inflammatory factors interleukin‑6 and tumor necrosis factor‑α by suppressing NF‑κB activation. In summary, the present study revealed that TIM‑3 displayed a regulatory role during the PDGF‑BB‑induced inflammatory reaction in HASMCs, which indicated that TIM‑3 may display anti‑atherosclerotic effects.
Databáze: OpenAIRE
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