Metallothionein production is a common tolerance mechanism in four species growing in polluted Cu mining areas in Peru

Autor: Sofía Ángela Portilla Llerena, Juan Pablo Portilla Llerena, Raúl Lima Coasaca, Ysabel Diaz Valencia, Paulo Mazzafera, Herbert Omar Lazo Rodriguez
Rok vydání: 2020
Předmět:
Chlorophyll
Proteomics
Health
Toxicology and Mutagenesis
0211 other engineering and technologies
Adaptation
Biological
02 engineering and technology
Growth
010501 environmental sciences
01 natural sciences
Plant Roots
Environmental pollution
Antioxidants
chemistry.chemical_compound
Peru
Soil Pollutants
GE1-350
Biomass
Carotenoid
chemistry.chemical_classification
biology
Chenopodium
Phytoextraction
General Medicine
Pollution
Horticulture
Biodegradation
Environmental
TD172-193.5
Catalase
Environmental Pollutants
Plant Development
Photosynthesis
Mining
Superoxide dismutase
PROTEÍNAS DE PLANTAS
Species Specificity
0105 earth and related environmental sciences
021110 strategic
defence & security studies
Protein
Public Health
Environmental and Occupational Health
biology.organism_classification
Rhizome
Environmental sciences
Phytoremediation
chemistry
Seedlings
biology.protein
Protein expression
Metallothionein
Tolerance
Copper
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Ecotoxicology and Environmental Safety, Vol 212, Iss, Pp 112009-(2021)
ISSN: 1090-2414
Popis: Cu pollution is a problem in mining areas in Peru. Here we evaluate the phytoextraction capacity, physiological and proteomic responses of four species growing in copper-contaminated areas in Arequipa, Peru. The plants used in the experiments were obtained by collecting seedlings (Tessaria integrifolia, Bacharis salicifolia), rhizomes (Eleocharis montevidensis) and seeds (Chenopodium murale) along a polluted river. They were exposed to solutions containing 2, 4, 8, 16 and 32 mg Cu L-1 during 20 days. Growth was affected in a concentration-dependent way. According to the tolerance index, B. salicifolia and C. murale were the most sensitive species, but with greater Cu phytoextraction capacity and accumulation in the biomass. The content and ratio of photosynthetic pigments changed differently for each specie and carotenoids level were less affected than chlorophyll. Cu also induced changes in the protein and sugar contents. Antioxidant enzyme activities (catalase and superoxide dismutase) increased with a decrease in the malondialdehyde. There were marked changes in the protein 2D-PAGE profiles with an increase in the abundance of metallothioneins (MT) of class II type I and II. Our results suggest that these species can grow in Cu polluted areas because they developed multiple tolerance mechanisms, such as and MTs production seems a important one.
Databáze: OpenAIRE
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