Ultrastructural basis for the transition of cell death mode from apoptosis to necrosis in menadione-treated osteosarcoma 143B cells
| Autor: | Makoto Masaoka, Mariusz Karbowski, Takashi Wakabayashi, Jiro Usukura, Jakub Kedzior, Marcin Kamiński, Yuji Nishizawa |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Programmed cell death
Time Factors Necrosis Apoptosis Biology Flow cytometry chemistry.chemical_compound Menadione Annexin Antifibrinolytic agent Tumor Cells Cultured medicine Humans Propidium iodide Instrumentation Cell Nucleus Osteosarcoma medicine.diagnostic_test Cell Membrane Vitamin K 3 Flow Cytometry Molecular biology Antifibrinolytic Agents Mitochondria Microscopy Electron chemistry medicine.symptom |
| Zdroj: | Journal of Electron Microscopy. 52:313-325 |
| ISSN: | 1477-9986 0022-0744 |
| DOI: | 10.1093/jmicro/52.3.313 |
| Popis: | Time-dependent ultrastructural changes of menadione-treated human osteosarcoma 143B cells were correlated with those in their stainability to Annexin V and propidium iodide (PI). Populations of both apoptotic (Annexin V(+)/PI(-)) and necrotic (Annexin V(+)/PI(+)) cells, judged by flow cytometry, began to increase at 2 h after menadione treatment. The former reached a maximum at 6 h followed by abrupt decreases thereafter, while the latter continued to increase. Electron microscopically, cells obtained at 6 h after the menadione treatment consisted of mixed populations of cells with typical apoptotic features and those with a mixture of apoptotic and necrotic features, while cells obtained at 8-24 h consisted exclusively of cells with a mixture of apoptotic and necrotic features. Thus, necrotic cells, as judged by flow cytometry, were in a transitional state of cell death mode from apoptosis to necrosis and are thus designated as 'intermediate cells'. Lack of apoptotic bodies, judged by flow cytometric analysis on sub-G1 nuclei and by electron microscopy in menadione-treated cells, suggested that the transition of cell death mode from apoptosis to necrosis occurred before the apoptotic processes were completed. Effects of N-acetylcysteine and Z-VAD-fmk on menadione-induced ultrastructural changes were also studied. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|