Identification of distinct hepatic and pulmonary forms of microsomal flavin-containing monooxygenase in the mouse and rabbit
| Autor: | Ernest Hodgson, Ronald E. Tynes, Patrick J. Sabourin |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Male
Immunodiffusion Cytochrome Swine Biophysics Flavin-containing monooxygenase Biology Biochemistry Mice Microsomes Animals Lung Molecular Biology chemistry.chemical_classification Mice Inbred ICR Cell Biology Metabolism Hydrogen-Ion Concentration respiratory system Monooxygenase Ouchterlony double immunodiffusion Enzyme chemistry Microsomes Liver Oxygenases Microsome biology.protein Female Rabbits |
| Zdroj: | Biochemical and Biophysical Research Communications. 126:1069-1075 |
| ISSN: | 0006-291X |
| Popis: | The flavin-containing monooxygenase has been purified from mouse and rabbit lung microsomes and shown to be distinct from the flavin-containing monooxygenase found in the liver of the same species. The mouse and rabbit lung monooxygenases have a unique ability to N-oxidize the primary aliphatic amine, n-octylamine, commonly included in microsomal incubations to inhibit cytochrome P-450. In the mouse lung, this compound not only serves as a substrate but is also a positive effector of metabolism. The mouse and rabbit lung enzymes have unusual pH optimum, near 9.8, compared to the liver enzymes which have peaks near pH 8.8. Using antibodies raised in goats, Ouchterlony immunodiffusion analysis indicates that the liver and lung proteins are immunochemically dissimilar. |
| Databáze: | OpenAIRE |
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