Genome size and organization in the blacklegged tick, Ixodes scapularis and the Southern cattle tick, Boophilus microplus
| Autor: | C. M. R. Lima, Felix D. Guerrero, Joseph Piesman, Amy J. Ullmann, W. C. Black |
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| Rok vydání: | 2005 |
| Předmět: |
Ixodidae
Period (gene) Biology Tick Genome chemistry.chemical_compound Genome Components Species Specificity Genetics Animals Repeated sequence Molecular Biology Genome size Repetitive Sequences Nucleic Acid Chromatography Foldback (sound engineering) DNA biology.organism_classification Virology Molecular biology Kinetics chemistry Ixodes scapularis Insect Science Nucleic Acid Renaturation Regression Analysis |
| Zdroj: | Insect molecular biology. 14(2) |
| ISSN: | 0962-1075 |
| Popis: | Genome sizes and the organization of repetitive DNA were determined in the hard ticks Ixodes scapularis and Boophilus microplus using reassociation kinetics. The I. scapularis genome contains approximately 2.15 pg (2.1x10(3) Mbp) of DNA and consists of no foldback (FB), 27% highly repetitive (HR), 39% moderately repetitive (MR), and 34% unique DNA. The B. microplus genome contains 7.5 pg (7.1x10(3) Mbp) DNA, and consists of 0.82% FB, 31% HR, 38% MR, and 30% unique DNA. In both species, repetitive sequences occur in a mixture of long and short period interspersion but most (65-80%) of the DNA follows a pattern of short period interspersion. Genome size and organization in the three tick species so far examined are distinct from other arthropods in having a greater proportion of MR, a lower proportion of unique and HR DNA of very low sequence complexity. |
| Databáze: | OpenAIRE |
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