Lung antioxidant enzymes are regulated by development and increased pulmonary blood flow
| Autor: | Shruti Sharma, Albert Grobe, Sanjiv Kumar, Anthony Azakie, Eileen Benavidez, Jeffrey R. Fineman, Peter Oishi, Ida Najwer, Stephen M. Black, Manal Englaish, Dean A. Wiseman |
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| Rok vydání: | 2007 |
| Předmět: |
Pulmonary and Respiratory Medicine
Aging Pulmonary Circulation Antioxidant Physiology medicine.medical_treatment Pharmacology Pulmonary Artery medicine.disease_cause Superoxide dismutase Superoxide Dismutase-1 Superoxides Physiology (medical) medicine.artery medicine Animals Tissue Distribution Lung chemistry.chemical_classification Reactive oxygen species Sheep biology Superoxide Dismutase Electron Spin Resonance Spectroscopy Cell Biology Hydrogen Peroxide Catalase Immunohistochemistry medicine.anatomical_structure chemistry Circulatory system Immunology Pulmonary artery biology.protein Oxidoreductases Oxidative stress |
| Zdroj: | American journal of physiology. Lung cellular and molecular physiology. 293(4) |
| ISSN: | 1040-0605 |
| Popis: | Increasing data suggest that oxidative stress, due to an increased production of reactive oxygen species and/or a decrease in antioxidants, is involved in the pathophysiology of pulmonary hypertension. Several antioxidant systems regulate the presence of oxidant species in vivo, and of primary interest are the superoxide dismutases (SOD) and catalase. However, little is known about the expression of antioxidant enzymes during the development of pulmonary hypertension. This study uses our lamb model of increased postnatal pulmonary blood flow, secondary to in utero aortopulmonary graft placement (shunt lambs), to investigate the expression patterns as well as activities of antioxidant enzymes during the early development of pulmonary hypertension. Protein levels of catalase, SOD1, SOD2, and SOD3 were evaluated by Western blot, and the activities of catalase and SOD were also quantified. In control lambs, protein expression and activities of catalase and SOD2 increased postnatally ( P < 0.05). However, SOD1 and SOD3 protein levels did not change. In shunt lambs, catalase, SOD1, and SOD2 protein levels all increased over the first 8 wk of life ( P < 0.05). However, SOD3 did not change. This was associated with an increase in the activities of catalase and SOD2 ( P < 0.05). Compared with control lambs, catalase and SOD2 protein levels were decreased in 2-wk-old shunt lambs and this was associated with increased levels of hydrogen peroxide (H2O2) and superoxide ( P < 0.05). Developmentally superoxide but not H2O2 levels significantly increased in both shunt and control lambs with levels being significantly higher in shunt compared with control lambs at 2 and 4 but not 8 wk. These data suggest that the antioxidant enzyme systems are dynamically regulated postnatally, and this regulation is altered during the development of pulmonary hypertension secondary to increased pulmonary blood flow. An increased understanding of these alterations may have important therapeutic implications for the treatment of pulmonary hypertension secondary to increased pulmonary blood flow. |
| Databáze: | OpenAIRE |
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