A Single Cysteine Residue in the Translocation Pathway of the Mitosomal ADP/ATP Carrier from Cryptosporidium parvum Confers a Broad Nucleotide Specificity
| Autor: | King, Martin S., Tavoulari, Sotiria, Mavridou, Vasiliki, King, Alannah C., Mifsud, John, Kunji, Edmund R. S. |
|---|---|
| Přispěvatelé: | King, Martin S. [0000-0001-6030-5154], Tavoulari, Sotiria [0000-0002-4263-8905], Mavridou, Vasiliki [0000-0002-1019-1256], King, Alannah C. [0000-0003-4100-3657], Mifsud, John [0000-0002-9647-6948], Kunji, Edmund R. S. [0000-0002-0610-4500], Apollo - University of Cambridge Repository, King, Martin S [0000-0001-6030-5154], King, Alannah C [0000-0003-4100-3657], Kunji, Edmund RS [0000-0002-0610-4500] |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Models Molecular Atractyloside Substrate Specificity lcsh:Chemistry Serine chemistry.chemical_compound 0302 clinical medicine Nucleotide adenine nucleotide translocase substrate specificity and selectivity lcsh:QH301-705.5 Spectroscopy Phylogeny chemistry.chemical_classification biology Nucleotides Adenine nucleotide translocator General Medicine Computer Science Applications Transport protein Mitochondria Lactococcus lactis Biochemistry substrate binding site adenine nucleotide translocator SLC25 mitochondrial carrier family Protein Translocation Systems ATP–ADP translocase Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae Catalysis Article Inorganic Chemistry 03 medical and health sciences Structure-Activity Relationship Amino Acid Sequence Cysteine Physical and Theoretical Chemistry Molecular Biology Cryptosporidium parvum Organic Chemistry Mitochondrial carrier Uridine 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 chemistry biology.protein Mutant Proteins Bongkrekic Acid Mitochondrial ADP ATP Translocases 030217 neurology & neurosurgery |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 21; Issue 23; Pages: 8971 International Journal of Molecular Sciences, Vol 21, Iss 8971, p 8971 (2020) |
| ISSN: | 1422-0067 |
| Popis: | Cryptosporidiumparvum is a clinically important eukaryotic parasite that causes the disease cryptosporidiosis, which manifests with gastroenteritis-like symptoms. The protist has mitosomes, which are organelles of mitochondrial origin that have only been partially characterized. The genome encodes a highly reduced set of transport proteins of the SLC25 mitochondrial carrier family of unknown function. Here, we have studied the transport properties of one member of the C. parvum carrier family, demonstrating that it resembles the mitochondrial ADP/ATP carrier of eukaryotes. However, this carrier has a broader substrate specificity for nucleotides, transporting adenosine, thymidine, and uridine di- and triphosphates in contrast to its mitochondrial orthologues, which have a strict substrate specificity for ADP and ATP. Inspection of the putative translocation pathway highlights a cysteine residue, which is a serine in mitochondrial ADP/ATP carriers. When the serine residue is replaced by cysteine or larger hydrophobic residues in the yeast mitochondrial ADP/ATP carrier, the substrate specificity becomes broad, showing that this residue is important for nucleotide base selectivity in ADP/ATP carriers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|