The Microbiome in Paediatric Crohn's Disease-A Longitudinal, Prospective, Single-Centre Study
| Autor: | Josef Wagner, Carl D. Kirkwood, Sarah Thomas, Anthony G. Catto-Smith, Mark R. Oliver, George Alex, Shivani Kansal, Donald J. S. Cameron, Karen Boniface |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Adolescent Biopsy Faecalibacterium prausnitzii Inflammatory bowel disease Gastroenterology Cohort Studies Intestinal mucosa Crohn Disease Internal medicine Medicine Humans Microbiome Longitudinal Studies Prospective Studies Intestinal Mucosa Prospective cohort study Child Crohn's disease Clostridiales biology business.industry Gastrointestinal Microbiome Australia Patient Acuity General Medicine Fusobacterium medicine.disease biology.organism_classification Female business |
| Zdroj: | Journal of Crohn'scolitis. 13(8) |
| ISSN: | 1876-4479 |
| Popis: | Background and AimsThe gut mucosa is the principal site where Crohn’s disease [CD] inflammation occurs. Limited information is available about the gut mucosal microbiome during CD relapse and remission. The aim of our study was to characterize specific changes in the gut microbiome during relapse and remission in a large single-centre paediatric CD cohort.MethodsWe analysed the microbiome of 345 biopsies from 204 patients, including 88 CD first diagnosis [CDFD] patients, 38 relapse [CDRL] patients, 12 remission [CDRM] patients, and 66 controls. Species identification was conducted using oligotyping in combination with ARB/SILVA taxonomic annotation.ResultsWe observed 45 bacteria to differ between CDFD samples and controls with statistical significance, with Fusobacterium being the most implicated species in CDFD patients. We also identified gender-specific differences in CD. Five species showed a strong association with CDRL patients and 10 species with CDRM patients. Three taxa showed a positive co-occurrence across the two groups. Hespellia porcina [closest taxonomic neighbour to Clostridium oroticum] was the most strongly associated with CDRL samples. Interestingly, Fusobacterium was not part of the CDRL-associated taxa group. Faecalibacterium prausnitzii was equally present in CDFD and control samples.ConclusionThis is the first study that has investigated the gut mucosal microbiome in a paediatric CD cohort with longitudinal sampling. Importantly, the microbiome of patients in CDRM did not return to a healthy control state. Neither did the microbiome of patients with CDRL return to the profile seen at CDFD. |
| Databáze: | OpenAIRE |
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