A real-time impedance based method to assess Rhodococcus equi virulence
| Autor: | Wim G. Meijer, Haixia Luo, Nora T. Lieggi, Jeremy C. Simpson, Raúl Miranda-CasoLuengo, Aleksandra A. Miranda-CasoLuengo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Anatomy and Physiology
animal diseases Veterinary Microbiology lcsh:Medicine Mice Plasmid Multiplicity of infection Electricity Immune Physiology Molecular Cell Biology Electric Impedance Rhodococcus equi lcsh:Science Pathogen 0303 health sciences Multidisciplinary biology Physics Bacterial Pathogens Host-Pathogen Interaction Host-Pathogen Interactions Cellular Types Actinomycetales Infections Intracellular Research Article Immune Cells Immunology Virulence Microbiology Cell Line 03 medical and health sciences Animals Humans Horses Biology Microbial Pathogens 030304 developmental biology Gram Positive 030306 microbiology Intracellular parasite Macrophages lcsh:R Electric Conductivity biology.organism_classification Virology Mutation Veterinary Science lcsh:Q Bacteria |
| Zdroj: | PLoS ONE, Vol 8, Iss 3, p e60612 (2013) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Rhodococcus equi is a facultative intracellular pathogen of macrophages and the causative agent of foal pneumonia. R. equi virulence is usually assessed by analyzing intracellular growth in macrophages by enumeration of bacteria following cell lysis, which is time consuming and does not allow for a high throughput analysis. This paper describes the use of an impedance based real-time method to characterize proliferation of R. equi in macrophages, using virulent and attenuated strains lacking the vapA gene or virulence plasmid. Image analysis suggested that the time-dependent cell response profile (TCRP) is governed by cell size and roundness as well as cytoxicity of infecting R. equi strains. The amplitude and inflection point of the resulting TCRP were dependent on the multiplicity of infection as well as virulence of the infecting strain, thus distinguishing between virulent and attenuated strains. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|