Brain Fatty Acid Composition and Inflammation in Mice Fed with High-Carbohydrate Diet or High-Fat Diet
| Autor: | Lorena Gimenez da Silva-Santi, Marina Masetto Antunes, Marco Mori, Camila Biesdorf de Almeida-Souza, Jesuí Vergílio Visentainer, Fabiana Carbonera, Amanda Rabello Crisma, Laureane Nunes Masi, Sandro Massao Hirabara, Rui Curi, Roberto Barbosa Bazotte |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine lcsh:TX341-641 Diet High-Fat Article Mice 03 medical and health sciences 0302 clinical medicine nutrients Dietary Carbohydrates Animals saturated fatty acids RNA Messenger Inflammation CD11b Antigen Nutrition and Dietetics Calcium-Binding Proteins Fatty Acids Microfilament Proteins monounsaturated fatty acids Brain food and beverages Antigens Differentiation Dietary Fats cytokines n-6/n-3 fatty acid ratio 030104 developmental biology Cyclooxygenase 2 lipids (amino acids peptides and proteins) Diet Carbohydrate Loading Nitric Oxide Synthase Integrin alpha Chains lcsh:Nutrition. Foods and food supply 030217 neurology & neurosurgery Food Science polyunsaturated fatty acids |
| Zdroj: | Nutrients Volume 10 Issue 9 Nutrients, Vol 10, Iss 9, p 1277 (2018) |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu10091277 |
| Popis: | Both high fat diet (HFD) and high carbohydrate diet (HCD) modulate brain fatty acids (FA) composition. Notwithstanding, there is a lack of information on time sequence of brain FA deposition either for HFD or HCD. The changes in brain FA composition in mice fed with HFD or HCD for 7, 14, 28, or 56 days were compared with results of 0 (before starting given the diets). mRNA expressions of allograft inflammatory factor 1 (Aif1), cyclooxygenase-2 (Cox 2), F4/80, inducible nitric oxide synthase (iNOS), integrin subunit alpha m (Itgam), interleukin IL-1&beta (IL-1&beta ), IL-6, IL-10, and tumor necrosis factor alpha (TNF-&alpha ) were measured. The HFD group had higher speed of deposition of saturated FA (SFA), monounsaturated FA (MUFA), and polyunsaturated FA (PUFA) at the beginning of the experimental period. However, on day 56, the total amount of SFA, MUFA, and PUFA were similar. mRNA expressions of F4/80 and Itgam, markers of microglia infiltration, were increased (p < 0.05) in the brain of the HCD group whereas inflammatory marker index (IMI) was higher (46%) in HFD group. In conclusion, the proportion of fat and carbohydrates in the diet modulates the speed deposition of FA and expression of inflammatory gene markers. |
| Databáze: | OpenAIRE |
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