Surface Plasmon Resonance (SPR) as a Tool for Antibody Conjugate Analysis
| Autor: | Kevin R. Shreder, Phillip G. Mattingly, Maciej Adamczyk, Zhiguang Yu |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.drug_class
Biomedical Engineering Biotin Pharmaceutical Science Bioengineering Biosensing Techniques Monoclonal antibody Binding Competitive medicine Reactivity (chemistry) Surface plasmon resonance Pharmacology Chromatography biology Chemistry Organic Chemistry Antibodies Monoclonal Antibody conjugate Surface Plasmon Resonance Solutions Dissociation constant biology.protein Biophysics Acridines Fluorescein Antibody Biotechnology Conjugate |
| Zdroj: | Bioconjugate Chemistry. 10:1032-1037 |
| ISSN: | 1520-4812 1043-1802 |
| Popis: | Surface plasmon resonance (SPR) analysis was used to assess the immunoreactivity of anti-biotin (4) and anti-fluorescein (5) monoclonal antibody after conjugation with the N-hydroxysuccinimide ester of acridinium-9-carboxamide 1. Only minor changes in the apparent equilibrium dissociation constants of the antibody conjugates for their ligands resulted from the conjugation process. However, comparison of the initial binding rate of the conjugates with their ligands with those of the unmodified antibodies over a range of concentrations showed that the antibody conjugates were partially inactivated. The anti-fluorescein conjugates retained at least 90% of their immunoreactivity over the range of modification tested, while anti-biotin conjugates showed a progressive loss of reactivity with increased substitution by the label. |
| Databáze: | OpenAIRE |
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