Transforming growth factor-β increases the expression of vascular smooth muscle cell Markers in human multi-lineage progenitor cells
| Autor: | Sarah M. Weakley, Changyi Chen, Peter H. Lin, Qizhi Yao, Hui Yang, Lidong Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Vascular smooth muscle
Cell Myocytes Smooth Muscle CD146 Antigen Biology Stem cell marker Muscle Smooth Vascular 03 medical and health sciences 0302 clinical medicine Tissue engineering Transforming Growth Factor beta medicine Myocyte Humans Cell Lineage RNA Messenger Progenitor cell smooth muscle cell 030304 developmental biology 0303 health sciences transforming growth factor β1 Stem Cells Endothelial Cells General Medicine Transforming growth factor beta differentiation Cell biology medicine.anatomical_structure Basic Research Gene Expression Regulation 030220 oncology & carcinogenesis multi-lineage progenitor cell cardiovascular system biology.protein Biomarkers Transforming growth factor |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 1234-1010 |
| Popis: | Summary Background Vascular smooth muscle cell (SMC) differentiation is an essential component of vascular repair and tissue engineering. However, currently used cell models for the study of SMC differentiation have several limitations. Multi-lineage progenitor cells (MLPCs) originate from human umbilical cord blood and are cloned from a single cell. The object of this study was to investigate whether MLPCs could differentiate into SMCs in vitro with induction by transforming growth factor β1 (TGF-β1). Material/Methods MLPCs were treated without or with TGF-β1 (1 and 5 ng/mL) in mesenchymal stem cell media plus 1% FBS for 7 days. Total RNA was isolated from the MLPCs, and semi-quantitative real-time PCR was performed to test the following mRNA levels: early and late phase SMC-specific markers, two endothelial cell (EC)-specific markers, endothelial progenitor cell (EPC) marker CD34, TGF-β1 accessory protein CD105, and adhesion molecule CD146. Results TGF-β1 (1 ng/mL) significantly increased the mRNA levels of SMC-specific markers SM22α, calponin-1, SM α-actin, caldesmon, tropomyosin and MLCK as well as adhesion molecule CD146. The mRNA levels of EC-specific markers VE-cadherin and VEGFR-2, EPC marker CD34 and TGF-β1 accessory protein CD105 were decreased significantly, after MLPC were treated with TGF-β1 (1 ng/mL). TGF-β1 at 5 ng/mL showed similar effect on the expression of these genes. Conclusions This study demonstrates that in the presence of TGF-β1, MLPCs undergo SMC lineage differentiation indicating that MLPCs are a promising cell model for SMC lineage differentiation studies, which may contribute to advances in vascular repair and tissue engineering. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|