Chronic mercury at low doses impairs white adipose tissue plasticity
| Autor: | Patricia Corrales, Gema Medina-Gómez, Franck Maciel Peçanha, Dalton Valentim Vassallo, Janaina Piagette, Giulia Alessandra Wiggers, Danize Aparecida Rizzetti, Marta Miguel, José Antonio Uranga-Ocio |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Ministerio de Ciencia e Innovación (España), Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Consejo Superior de Investigaciones Científicas (España) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Blood Glucose
Male 0301 basic medicine medicine.medical_specialty Adipose Tissue White Lipolysis medicine.medical_treatment Glucose uptake Adipocytes White Cell Plasticity Adipokine Apoptosis White adipose tissue Carbohydrate metabolism Toxicology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Adipocyte medicine Animals Insulin Rats Wistar Lipid and glucose metabolism Adiponectin Lipogenesis Mercury Lipids 030104 developmental biology Endocrinology Gene Expression Regulation chemistry Mercuric Chloride Endoplasmic reticulum stress Energy Metabolism 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | [Introduction]: The toxic effects of mercury (Hg) are involved in homeostasis of energy systems such as lipid and glucose metabolism, and white adipose tissue dysfunction is considered as a central mechanism leading to metabolic disorders. Objective: The aim of this study was to determine the effects of chronic inorganic Hg exposure at low doses on the lipid and glycemic metabolism. [Methods]: Male Wistar rats were divided into two groups and treated for 60 days with: saline solution, i.m. (Untreated) and mercury chloride, i.m. - 1st dose 4.6 μg/kg, subsequent doses 0.07 μg/kg/day - (Mercury). Histological analyses, Hg levels measurement and GRP78, CHOP, PPARα, PPARγ, leptin, adiponectin and CD11 mRNA expressions were performed in epididymal white adipose tissue (eWAT). Glucose, triglycerides, total cholesterol and insulin plasma levels were also measured. [Results]: Hg exposure reduced the absolute and relative eWAT weights, adipocyte size, plasma insulin levels, glucose tolerance, antioxidant defenses and increased plasma glucose and triglyceride levels. In addition, CHOP, GRP78, PPARα, PPARγ, leptin and adiponectin mRNA expressions were increased in Hg-treated animals. No differences in Hg concentration were found in eWAT between the untreated and Hg groups. These results suggest that the reduction in adipocyte size is related to the impaired antioxidant defenses, endoplasmic reticulum (ER) stress, the disrupted PPARs and adipokines mRNA expression induced by the metal in eWAT. These disturbances possibly induced a decrease in circulating insulin levels, an imbalance between lipolysis and lipogenesis mechanisms in eWAT, with an increase in fatty acids mobilization, a reduction in glucose uptake and an activation of pro-apoptotic pathways, leading to hyperglycemia and hyperlipidemia. [Conclusions]: Hg is a powerful environmental WAT disruptor that influences signaling events and impairs metabolic activity and hormonal balance of adipocytes. This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq [203440/2014-5] and the Ministerio de Economía y Competitividad – MINECO [AGL2012-32387 and CSIC – Intramural 201570I028]. |
| Databáze: | OpenAIRE |
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