Coating liposomes with collagen (Mr 50 000) increases uptake into liver
| Autor: | M. J. Fonseca, Francesca Reig, M.A. Alsina |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Collagen-coated liposome Phospholipid Biophysics Coated vesicle Biochemistry Mice chemistry.chemical_compound Pharmacokinetics Animals Tissue Distribution Rats Wistar Fluorescent Dyes Drug Carriers Liposome Chromatography Vesicle Liposome-protein interaction Half-life Cell Biology Fluoresceins In vitro Rats Molecular Weight Liver chemistry Liposomes Glycine Female Collagen Stability |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Biomembranes. 1279(2):259-265 |
| ISSN: | 0005-2736 |
| DOI: | 10.1016/0005-2736(95)00265-0 |
| Popis: | Collagen-coated small unilamellar liposomes were prepared by incubation of two hydrophobic derivatives of collagen (average Mr 50 000) with performed vesciles. The introduction of hexyl and lauryl residues to the collagen molecule improved by 10-fold the ability of collagen to coat liposomes. In vitro stability of the different coated vesicles prepared, was studied by their ability to retain entrapped carboxyfluorescein as a function of the time. Coated vesicles were clearly more stable in vitro than control liposomes, except for those containing the lauryl derivative in a protein/phospholipid weight ratio higher than 10−3. Vesicle clearance from circulation as well as tissue distribution were also determined. Pharmacokinetics (determined by both fluorescence and radioactive techniques) were highly dependent on the injected dose, phospholipids used and the content of collagen. Half-lives were maximum for liposomes composed of saturated phospholipids injected at a dose of 2 μmol phospholipid. Besides, blood elimination of collagen-containing vesicles was about 2-fold faster and liver uptake 1.5 to 2-fold higher than control liposomes. |
| Databáze: | OpenAIRE |
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