CD45-positive cells are not an essential component in cardiosphere formation
| Autor: | Richard E. Sievers, Yerem Yeghiazarians, Zhi-En Wang, Andrew J. Boyle, Jianqin Ye, Matthew Gormley, Henry Shih |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Cell type Histology Chemistry Cell Bone Marrow Cells Cell Biology Molecular biology Molecular medicine Pathology and Forensic Medicine Mice Inbred C57BL Cell therapy Mice Heterogeneous population medicine.anatomical_structure Spheroids Cellular Immunology otorhinolaryngologic diseases medicine Animals Leukocyte Common Antigens Myocytes Cardiac Bone marrow Progenitor cell Cell Proliferation |
| Zdroj: | Cell and Tissue Research. 351:201-205 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/s00441-012-1511-8 |
| Popis: | The cardiosphere (CS) is composed of a heterogeneous population of cells, including CD45(+) cells that are bone marrow (BM)-derived. However, whether the CD45(+) cells are an essential cell component in CS formation is unknown. The current study was undertaken to address this question. Cardiospheres (CSs) were harvested from 1-week post-myocardial infarction (MI) or non-MI hearts of C57BL/6 J mice. The process of CS formation was observed by timelapse photography. To analyze the role of BM-derived CD45(+) cells in CS formation, CD45(+) cells were depleted from populations of CS-forming cells by immunomagnetic beads. We recorded the number of CSs formed in culture from the same amount (10(5)) of intact CS-forming cells, from CD45(+)-cell-depleted CS-forming cells and from CD45(+) cells alone (n=6-9/cell type). CS-forming cells selectively aggregated together to form CSs by 35 h after plating. The depletion of CD45(+) cells from CS-forming cells actually increased the formation of CSs (67±10 CSs/10(5) cells) compared with non-depleted CS-forming cells (51±6 CSs/10(5) cells, P0.0001). Purified CD45(+) cells from CS-forming cells did not form CSs in culture. Thus, BM-derived CD45(+) cells including BM progenitors are neither necessary nor sufficient for CS formation. |
| Databáze: | OpenAIRE |
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