Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasis
| Autor: | Kris Morreel, Jean-Pierre Renou, Enrico Martinoia, Deborah Goffner, Urs Fischer, Stephanie Pfrunder, Benoît Lacombe, Philippe Ranocha, Yves Martinez, Claire Corratgé-Faillie, Jean-Baptiste Thibaud, Ondřej Novák, Judith Felten, Oana Dima, Karin Ljung, Réka Nagy, Wout Boerjan, Xu Jin |
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| Přispěvatelé: | University of Zurich, Goffner, Deborah, Surfaces Cellulaires et Signalisation chez les Végétaux (SCSV), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Department of Plant Systems Biology, Ghent University [Belgium] (UGENT)-Department of Plant Biotechnology and Bioinformatics, Institut of Plant Biology, University of Zürich [Zürich] (UZH), Umea Plant Science Center, Swedish University of Agricultural Sciences (SLU)-Department of Forest Genetics and Plant Physiology, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Universiteit Gent = Ghent University [Belgium] (UGENT)-Department of Plant Biotechnology and Bioinformatics, Universität Zürich [Zürich] = University of Zurich (UZH), Umea Plant Science Center (UPSC), Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences (SLU)-Swedish University of Agricultural Sciences (SLU), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Arabidopsis General Physics and Astronomy Vacuole 580 Plants (Botany) hormone auxin plasma membrane 01 natural sciences Xenopus laevis Plant Growth Regulators 10126 Department of Plant and Microbial Biology Cell Wall Gene Expression Regulation Plant Homeostasis Gene Regulatory Networks heterocyclic compounds évolution DISSECTION chemistry.chemical_classification 0303 health sciences Multidisciplinary biology BINDING PROTEIN-1 food and beverages 3100 General Physics and Astronomy Cell biology Fiber cell dissection plante endoplasmic reticulum membrane Plant hormone plant development Secondary cell wall Intracellular WALLS ARE THIN1 1600 General Chemistry Saccharomyces cerevisiae General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences ROOT Auxin 1300 General Biochemistry Genetics and Molecular Biology [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Animals PLANTS binding protein 1 polar transport plant gene identification thaliana root transcriptome evolution TRANSCRIPTOME 030304 developmental biology Endoplasmic reticulum membrane THALIANA POLAR TRANSPORT IDENTIFICATION Indoleacetic Acids Arabidopsis Proteins fungi arabidopsis thaliana auxin transport Biology and Life Sciences Membrane Transport Proteins Biological Transport General Chemistry biology.organism_classification GENE EVOLUTION racine chemistry Mutation Vacuoles 010606 plant biology & botany |
| Zdroj: | Nature Communications (4), . (2013) Nature Communications Nature Communications, Nature Publishing Group, 2013, 4, pp.2625. ⟨10.1038/ncomms3625⟩ NATURE COMMUNICATIONS |
| ISSN: | 2041-1723 |
| DOI: | 10.5167/uzh-87883 |
| Popis: | The plant hormone auxin (indole-3-acetic acid, IAA) has a crucial role in plant development. Its spatiotemporal distribution is controlled by a combination of biosynthetic, metabolic and transport mechanisms. Four families of auxin transporters have been identified that mediate transport across the plasma or endoplasmic reticulum membrane. Here we report the discovery and the functional characterization of the first vacuolar auxin transporter. We demonstrate that WALLS ARE THIN1 (WAT1), a plant-specific protein that dictates secondary cell wall thickness of wood fibres, facilitates auxin export from isolated Arabidopsis vacuoles in yeast and in Xenopus oocytes. We unambiguously identify IAA and related metabolites in isolated Arabidopsis vacuoles, suggesting a key role for the vacuole in intracellular auxin homoeostasis. Moreover, local auxin application onto wat1 mutant stems restores fibre cell wall thickness. Our study provides new insight into the complexity of auxin transport in plants and a means to dissect auxin function during fibre differentiation. The plant hormone auxin is essential for plant development and growth and is transported across cellular membranes via specialized transporter proteins. In this study, Ranocha et al. identify the first vacuolar auxin transporter, WAT1, suggesting an involvement of the vacuole in auxin signalling. |
| Databáze: | OpenAIRE |
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