Multicellular Spheroids Formation on Hydrogel Enhances Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells Under Magnetic Nanoparticles Induction
| Autor: | Yuqin Li, Ning Gu, Lin Wang, Ruhan Yan, Mengping Li, Shijia Tang, Zhaobin Guo, Ke Hu, Xueqin Xu, Tingting Yu, Sen Yan, Feimin Zhang, Xiao Han |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
tunable mechanical properties
Biophysics Pharmaceutical Science Bioengineering Biomaterials stomatognathic system International Journal of Nanomedicine Osteogenesis Dental pulp stem cells Spheroids Cellular Drug Discovery Humans multicellular spheroids Bone regeneration Magnetite Nanoparticles Cells Cultured Dental Pulp Original Research Cell Proliferation Chemistry Regeneration (biology) Stem Cells Organic Chemistry Spheroid Cell Differentiation Hydrogels General Medicine magnetic nanomaterials dental pulp stem cells Cell biology Self-healing hydrogels Alkaline phosphatase CD146 Stem cell osteogenic/odontogenic differentiation |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 1176-9114 |
| Popis: | Xiao Han,1,2 Shijia Tang,1 Lin Wang,1 Xueqin Xu,2 Ruhan Yan,2 Sen Yan,3 Zhaobin Guo,4 Ke Hu,1,2 Tingting Yu,5 Mengping Li,2 Yuqin Li,2 Feimin Zhang,1,2 Ning Gu2,3 1Jiangsu Key Laboratory of Oral Diseases, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, Jiangsu, Peopleâs Republic of China; 2Laboratory of Oral Regenerative Medicine Technology, School of Biomedical Engineering and Informatics, Department of Biomedical Engineering, Nanjing Medical University, Nanjing, Jiangsu, Peopleâs Republic of China; 3Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu, Peopleâs Republic of China; 4Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA; 5Department of Medical Genetics, School of Basic Medical Science & Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, Nanjing, Jiangsu, Peopleâs Republic of ChinaCorrespondence: Feimin Zhang; Ning Gu Fax +86-25-86516414; +86-25-83272460Email fmzhang@njmu.edu.cn; guning@seu.edu.cnIntroduction: Promotion odontogenic differentiation of dental pulp stem cells (DPSCs) is essential for dentin regeneration. Physical cellular microenvironment is of critical importance for stem cells differentiation and influences the function of other biological/chemical factors to differentiation.Methods: Based on adjusting the mechanical/interfacial properties of hydrogels, multicellular spheroids (MCSs) of DPSCs generated through self-organization. The spheroids were characterized by immunofluorescent staining and flow cytometry. Quantitative real-time polymerase chain reaction, alkaline phosphatase (ALP) activity assay, ALP staining and Alizarin Red S staining were performed to evaluate the osteogenic/odontogenic differentiation of DPSCs with or without magnetic iron oxide nanoparticles (IONPs) induction.Results: MCSs of DPSCs exhibited a significant upregulation of E-cadherin and N-cadherin and enriched CD146 positive subpopulation, along with a stronger osteogenic/odontogenic differentiation ability. Moreover, DPSCs spheroids showed more substantial osteogenic differentiation tendency than the classical two-dimensional cultured DPSCs under the stimulation of magnetic IONPs.Conclusion: Three-dimensional spheroids culture of DPSCs based on composite viscoelastic materials combined with mechanical/magnetic stimulation may provide a theoretical basis for the subsequent development of dentin or bone regeneration technology.Keywords: tunable mechanical properties, dental pulp stem cells, magnetic nanomaterials, osteogenic/odontogenic differentiation, multicellular spheroids |
| Databáze: | OpenAIRE |
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