The use of transgenics in the laboratory axolotl
| Autor: | Ji-Feng Fei, Prayag Murawala, Sofia-Christina Papadopoulos, Lydia Tilley, Marko Pende |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
ved/biology Transgene ved/biology.organism_classification_rank.species Solid base Computational biology Biology biology.organism_classification Ambystoma mexicanum Animals Genetically Modified 03 medical and health sciences Mice 030104 developmental biology 0302 clinical medicine Genome editing Axolotl Transgenic lines Animals Model organism Zebrafish 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental dynamics : an official publication of the American Association of Anatomists. 251(6) |
| ISSN: | 1097-0177 |
| Popis: | The ability to generate transgenic animals sparked a wave of research committed to implementing such technology in a wide variety of model organisms. Building a solid base of ubiquitous and tissue-specific reporter lines has set the stage for later interrogations of individual cells or genetic elements. Compared to other widely used model organisms such as mice, zebrafish and fruit flies, there are only a few transgenic lines available in the laboratory axolotl (Ambystoma mexicanum), although their number is steadily expanding. In this review, we discuss a brief history of the transgenic methodologies in axolotl and their advantages and disadvantages. Next, we discuss available transgenic lines and insights we have been able to glean from them. Finally, we list challenges when developing transgenic axolotl, and where further work is needed in order to improve their standing as both a developmental and regenerative model. |
| Databáze: | OpenAIRE |
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