High-throughput and clogging-free microfluidic filtration platform for on-chip cell separation from undiluted whole blood
| Autor: | Yinuo Cheng, Zengshuai Ma, Xie Shuai, Wenhui Wang, Xiongying Ye |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Fluid Flow and Transfer Processes
Chromatography Materials science Microfiltration 010401 analytical chemistry Microfluidics Biomedical Engineering Micropump 02 engineering and technology Microbead (research) 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences law.invention Colloid and Surface Chemistry Membrane law General Materials Science Sample collection 0210 nano-technology Filtration Regular Articles Whole blood |
| Zdroj: | Biomicrofluidics. 10:014118 |
| ISSN: | 1932-1058 |
| DOI: | 10.1063/1.4941985 |
| Popis: | Rapid separation of white blood cells from whole blood sample is often required for their subsequent analyses of functions and phenotypes, and many advances have been made in this field. However, most current microfiltration-based cell separation microfluidic chips still suffer from low-throughput and membrane clogging. This paper reports on a high-throughput and clogging-free microfluidic filtration platform, which features with an integrated bidirectional micropump and commercially available polycarbonate microporous membranes. The integrated bidirectional micropump enables the fluid to flush micropores back and forth, effectively avoiding membrane clogging. The microporous membrane allows red blood cells passing through high-density pores in a cross-flow mixed with dead-end filtration mode. All the separation processes, including blood and buffer loading, separation, and sample collection, are automatically controlled for easy operation and high throughput. Both microbead mixture and undiluted whole blood sample are separated by the platform effectively. In particular, for white blood cell separation, the chip recovered 72.1% white blood cells with an over 232-fold enrichment ratio at a throughput as high as 37.5 μl/min. This high-throughput, clogging-free, and highly integrated platform holds great promise for point-of-care blood pretreatment, analysis, and diagnosis applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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