The unusual UBZ domain of Saccharomyces cerevisiae polymerase η
| Autor: | Sanjay D'Souza, Rachel V. Woodruff, Graham C. Walker, Martha G. Bomar, Pei Zhou |
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| Přispěvatelé: | Massachusetts Institute of Technology. Department of Biology, Woodruff, Rachel V., D'Souza, Sanjay Victor, Walker, Graham C. |
| Rok vydání: | 2010 |
| Předmět: |
Ubiquitin binding
Molecular Sequence Data Saccharomyces cerevisiae Sequence alignment DNA-Directed DNA Polymerase Plasma protein binding Biochemistry Article Conserved sequence Mice Species Specificity Ubiquitin Animals Humans Molecular Biology Conserved Sequence Edetic Acid Polymerase Zinc finger Sequence Homology Amino Acid biology Zinc Fingers Cell Biology biology.organism_classification Protein Structure Tertiary Zinc Mutation Mutagenesis Site-Directed biology.protein Sequence Alignment Protein Binding |
| Zdroj: | PMC |
| ISSN: | 1568-7864 |
| DOI: | 10.1016/j.dnarep.2010.08.001 |
| Popis: | Recent research has revealed the presence of ubiquitin-binding domains in the Y family polymerases. The ubiquitin-binding zinc finger (UBZ) domain of human polymerase η is vital for its regulation, localization, and function. Here, we elucidate structural and functional features of the non-canonical UBZ motif of Saccharomyces cerevisiae pol η. Characterization of pol η mutants confirms the importance of the UBZ motif and implies that its function is independent of zinc binding. Intriguingly, we demonstrate that zinc does bind to and affect the structure of the purified UBZ domain, but is not required for its ubiquitin-binding activity. Our finding that this unusual zinc finger is able to interact with ubiquitin even in its apo form adds support to the model that ubiquitin binding is the primary and functionally important activity of the UBZ domain in S. cerevisiae polymerase η. Putative ubiquitin-binding domains, primarily UBZs, are identified in the majority of known pol η homologs. We discuss the implications of our observations for zinc finger structure and pol η regulation. National Institute of Environmental Health Sciences (Grant ES-015818) National Institute of Environmental Health Sciences (Grant P30 ES-002109) American Cancer Society (Research Professorship) |
| Databáze: | OpenAIRE |
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