Distribution of Phototrophic Purple Nonsulfur Bacteria in Massive Blooms in Coastal and Wastewater Ditch Environments
Autor: | Yo Kikuchi, So Umekage, Akira Hiraishi, Nobuyoshi Nagao, Chinatsu Yonekawa, Toshihiko Eki, Yuu Hirose |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
Microbiology (medical)
pufM gene food.ingredient Rhodovulum pufm gene massive blooms Microbiology Article 03 medical and health sciences food purple nonsulfur bacteria rhodovulum Virology Purple sulfur bacteria Organic matter Microbial mat lcsh:QH301-705.5 030304 developmental biology chemistry.chemical_classification 0303 health sciences Rhodobacter biology Phototroph 030306 microbiology phylogenomics Rhodopseudomonas biology.organism_classification Wastewater chemistry lcsh:Biology (General) Environmental chemistry anoxygenic phototrophic bacteria Environmental science |
Zdroj: | Microorganisms, Vol 8, Iss 2, p 150 (2020) Microorganisms Volume 8 Issue 2 |
ISSN: | 2076-2607 |
Popis: | The biodiversity of phototrophic purple nonsulfur bacteria (PNSB) in comparison with purple sulfur bacteria (PSB) in colored blooms and microbial mats that developed in coastal mudflats and pools and wastewater ditches was investigated. For this, a combination of photopigment and quinone profiling, pufM gene-targeted quantitative PCR, and pufM gene clone library analysis was used in addition to conventional microscopic and cultivation methods. Red and pink blooms in the coastal environments contained PSB as the major populations, and smaller but significant densities of PNSB, with members of Rhodovulum predominating. On the other hand, red-pink blooms and mats in the wastewater ditches exclusively yielded PNSB, with Rhodobacter, Rhodopseudomonas, and/or Pararhodospirillum as the major constituents. The important environmental factors affecting PNSB populations were organic matter and sulfide concentrations and oxidation−reduction potential (ORP). Namely, light-exposed, sulfide-deficient water bodies with high-strength organic matter and in a limited range of ORP provide favorable conditions for the massive growth of PNSB over co-existing PSB. We also report high-quality genome sequences of Rhodovulum sp. strain MB263, previously isolated from a pink mudflat, and Rhodovulum sulfidophilum DSM 1374T, which would enhance our understanding of how PNSB respond to various environmental factors in the natural ecosystem. |
Databáze: | OpenAIRE |
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