Relationship between hydroxycinnamic acids and the resistance of apple cultivars to rosy apple aphid

Autor: Diego Micheletti, María José Antón, Andrea Sasía-Arriba, Marcos Miñarro, Luis A. Berrueta, Rosa M. Alonso-Salces, Blanca Gallo, E. Dapena
Rok vydání: 2018
Předmět:
0106 biological sciences
0301 basic medicine
Spectrometry
Mass
Electrospray Ionization
Breeding program
Coumaric Acids
Otras Ciencias Biológicas
Plant Resistance
Plant resistance
Apple tree
Hydroxycinnamic acids
01 natural sciences
Analytical Chemistry
Ciencias Biológicas
Hemiptera
03 medical and health sciences
chemistry.chemical_compound
Dysaphis plantaginea
Settore AGR/13 - CHIMICA AGRARIA
Animals
Cultivar
Phenols
Least-Squares Analysis
Dysaphis Plantaginea
Chromatography
High Pressure Liquid
Aphid
biology
Phenylpropanoid
Chemistry
Malus Domestica
Ciencias Químicas
Hydroxycinnamic Acids
biology.organism_classification
On resistance
Phenolic compounds
Phenolic Compounds
Horticulture
030104 developmental biology
Malus domestica
Malus
Química Analítica
CIENCIAS NATURALES Y EXACTAS
010606 plant biology & botany
Zdroj: Talanta. 187
ISSN: 1873-3573
Popis: The phenolic profiles of apple cultivars from the SERIDA Asturian cider apple breeding program, including parents and progenies, were determined by ultrahigh-performance liquid chromatography-diode array detector-electrospray ionization-quadrupole time of flight/mass spectrometer in order to study the relationship between phenols and the resistance of apple tree cultivars to rosy apple aphid (RAA). A pattern recognition technique named partial least square discriminant analysis (PLS-DA) was used to classify apple cultivars based on resistance to RAA, resistant and susceptible, reaching scores with accuracy higher than 97% and 91% respectively. Hydroxycinnamic acids, particularly 4-caffeoylquinic acid (4-CQA) and 4-p-coumaroylquinic acid (4-pCoQA), were identified as the major player in RAA resistance by the PLS-DA model. Indeed, the isomerisation 5-CQA → 4-CQA is favoured in resistant cultivars, whereas the isomerisation 5-pCoQA → 4-pCoQA is favoured in susceptible cultivars. As a result, resistant cultivars accumulate higher amounts of 4-CQA than susceptible ones, and the opposite occurs for 4-pCoQA. Also, minor isomerisations of 5-CQA to 1-CQA or 3-CQA show opposite behaviour for resistant and susceptible cultivars. Cultivar resistance to RAA is concluded to be related with the phenylpropanoid pathway, the isomerisation reactions being the key metabolic reaction for a cultivar to be resistant or susceptible to RAA. Fil: Berrueta, Luis A.. Universidad del País Vasco; España Fil: Sasía-Arriba, Andrea. Universidad del País Vasco; España Fil: Miñarro, Marcos. Serida; España Fil: Antón, María J.. Serida; España Fil: Alonso Salces, Rosa Maria. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Biología. Laboratorio de Artrópodos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata; Argentina Fil: Micheletti, Diego. Instituto Agrario San Michele all'Adige Fondazione Edmund Mach; Italia Fil: Gallo, Blanca. Universidad del País Vasco; España Fil: Dapena, Enrique. Serida; España
Databáze: OpenAIRE
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