Engineering of functional tendon
| Autor: | Sarah Calve, Ellen M. Arruda, Robert G. Dennis, Paul E. Kosnik, Keith Baar, Karl Grosh |
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| Rok vydání: | 2004 |
| Předmět: |
musculoskeletal diseases
Scaffold Cell Culture Techniques Extracellular matrix Tendons Tensile Strength Ultimate tensile strength medicine Animals Cells Cultured Cell Proliferation Achilles tendon Tissue Engineering Chemistry General Engineering Fibroblasts musculoskeletal system Embryonic stem cell Elasticity Rats Inbred F344 Tendon Rats Transplantation medicine.anatomical_structure Animals Newborn Embryonic chick Biomedical engineering |
| Zdroj: | Tissue engineering. 10(5-6) |
| ISSN: | 1076-3279 |
| Popis: | Surgical tendon repair is limited by the availability of viable tissue for transplantation. Because of its relatively avascular nature, tendon is a prime candidate for engineered tissue replacement. To address this problem, cells isolated from rat Achilles tendon were grown to confluence in culture and allowed to self-assemble into a cylinder between two anchor points. The resulting scaffold-free tissue was composed of aligned, small-diameter collagen fibrils, a large number of cells, and an excess of noncollagenous extracellular matrix; all characteristics of embryonic tendon. The stress–strain response of the constructs also resembles the nonlinear behavior of immature tendons, and the ultimate tensile strength is approximately equal to that of embryonic chick tendon, roughly 2 MPa. These physical and mechanical properties indicate that these constructs are the first viable tendons engineered in vitro, without the aid of artificial scaffolding. |
| Databáze: | OpenAIRE |
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