Clostridium perfringens epsilon toxin is cytotoxic for human renal tubular epithelial cells
| Autor: | Claudia Silberstein, Mariano E. Fernandez Miyakawa, Osvaldo Zabal |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Pathology
medicine.medical_specialty Time Factors Cell Survival Health Toxicology and Mutagenesis Bacterial Toxins Biology Toxicology medicine.disease_cause Enterotoxemia medicine Humans Cytotoxic T cell Viability assay Cell damage Cells Cultured Kidney Dose-Response Relationship Drug Epithelial Cells General Medicine Clostridium perfringens Clostridium perfringens epsilon toxin medicine.disease Molecular biology Kidney Tubules medicine.anatomical_structure Cell culture Protein Binding |
| Zdroj: | Human & Experimental Toxicology. 30:275-282 |
| ISSN: | 1477-0903 0960-3271 |
| Popis: | Clostridium perfringens epsilon toxin (ETX) is responsible for a fatal enterotoxemia in different animal species, producing extensive renal damage, neurological disturbance and edema of lungs, heart and kidneys. However, there is no information about the susceptibility of humans to ETX. Here, we report that primary cultures of human renal tubular epithelial cells (HRTEC) exposed to ETX showed a marked swelling with subsequent large blebs surrounding most cells. The incubation of HRTEC with ETX produced a reduction of cell viability in a dose- and time-dependent manner. The CD50 after 1-hour and 24-hour incubation were 3 µg/mL and 0.5 µg/mL, respectively. The pulse with ETX for 3 min was enough to produce a significant cytotoxic effect on HRTEC after 1-hour incubation. ETX binds to HRTEC forming a large complex of about 160 kDa similar to what was found in the Madin-Darby canine kidney (MDCK) cell line. The HRTEC could be a useful cell model to improve the understanding of the mechanisms involved on the cell damage mediated by ETX. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|