Mitotic deletions of 11p15.5 in two different tumors indicate that the CALCA locus is distal to the PTH locus
| Autor: | S. Grandjouan, F. Barichard, C. Huerre-Jeanpierre, Claudine Junien, I. Henry |
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| Rok vydání: | 1989 |
| Předmět: |
Male
Calcitonin Gene-Related Peptide Mitosis Locus (genetics) Biology Wilms Tumor Genetics Carcinoma medicine Humans Adrenocortical carcinoma Allele Molecular Biology Genetics (clinical) Epithelioma Chromosomes Human Pair 11 Neuropeptides Chromosome Mapping Wilms' tumor DNA Neoplasm medicine.disease Adrenal Cortex Neoplasms Kidney Neoplasms Blotting Southern Parathyroid Hormone Genetic marker Cancer research Chromosome Deletion |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1424-859X 1424-8581 |
| DOI: | 10.1159/000132748 |
| Popis: | We have compared the constitutional and tumor genotypes in two patients with Wilms tumor and adrenocortical carcinoma. The allelic distribution of chromosome 11-specific markers spanning chromosome 11 from pter to qter (HRAS1-HBB-[CALCA/PTH]-FSHB-CAT-APOAl) and an approach combining RFLP analysis and gene copy number determination showed that a mitotic deletion had occurred in both tumors. The loss of one copy of the gene for α-calcitonin-gene-related poly peptide (CALCA), together with that of a more distal marker (HRASl or HBB), indicates that CALCA is distal to the gene for parathyroid hormone (PTH), which was not deleted in either tumor. These results suggest that mitotic deletion mapping may be as useful as meiotic deletion or recombination mapping in ordering closely linked markers, such as CALCA and PTH, for which other approaches, including physical mapping and multipoint linkage analysis, have failed to accurately identify the gene order. |
| Databáze: | OpenAIRE |
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