Comparison of in vitro pre-eclampsia (PE) cell models with PE placental tissue
| Autor: | Louise V. Briggs, Henning Schneider, Sampada Kallol, Hiten D. Mistry, Christiane Albrecht, Ruedi Moser |
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| Rok vydání: | 2019 |
| Předmět: |
Cell
Obstetrics and Gynecology Hypoxia (medical) medicine.disease_cause medicine.disease In vitro Pathophysiology Preeclampsia Andrology medicine.anatomical_structure Reproductive Medicine Downregulation and upregulation embryonic structures medicine 570 Life sciences biology Secretion medicine.symptom 610 Medicine & health Oxidative stress Developmental Biology |
| Zdroj: | Placenta. 83:e104 |
| ISSN: | 0143-4004 |
| DOI: | 10.1016/j.placenta.2019.06.329 |
| Popis: | Objectives: We induced hypoxia and oxidative stress (OS) in cytotrophoblasts (CTB) from control placentae to mimic the characteristics of preeclampsia (PE). For validation we compared the gene and protein expression of established PE biomarkers between the cell models and PE placentae. Methods: CTB isolated from healthy term placentae (n¼2) were cultured either under normoxic (5% pCO2;21% pO2;24h), hypoxic (5% pCO2;1.5% pO2;24h) or OS (consecutive 6h-intervals of normoxia and hypoxia until 24h) conditions. mRNA abundances of PE biomarkers (Table1) were analysed by qRT-PCR in the cell models and PE placentae (n¼12). Additionally, the secretion of sFlt1 and PlGF was determined by ELISA in cell culture supernatants. Statistical analysis was performed using unpaired t-test. Results: The cell models recapitulated important pathophysiological changes occurring in PE (Table1) including the upregulation of sFlt1 which was also observed in PE placentae. Interestingly, in the cell models the sFlt1/PlGF ratio was above the clinically applied cutoff value of 38 for hypoxic (77.3±35.2) and OS (124±88.3), but |
| Databáze: | OpenAIRE |
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