Contribution of Next-Generation Sequencing to Aquatic and Fish Virology
| Autor: | Laurent Bigarré, Andriniaina Andy Nkili-Meyong, Nicolas Berthet, Tatiana Vallaeys, Jean-Christophe Avarre, Ingrid Labouba |
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| Přispěvatelé: | Centre International de Recherches Médicales de Franceville (CIRMF), Laboratoire de Ploufragan-Plouzané-Niort [ANSES], Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Université de Montpellier (UM), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Epidémiologie et Physiopathologie des Virus Oncogènes / Oncogenic Virus Epidemiology and Pathophysiology (EPVO (UMR_3569 / U-Pasteur_3)), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats (CIBU), Institut Pasteur [Paris], Environnement et Risques infectieux - Environment and Infectious Risks (ERI), The CIRMF is supported by the government of Gabon, Total-Fina-Elf Gabon, and the Ministère de la Coopération Française. This review article was also made possible by the ERA-NET EMIDA-funded project MOLTRAQ (molecular tracing of viral pathogens in aquaculture)., Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut Pasteur [Paris] (IP) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Molecular epidemiology Genomics Diagnostics Next-generation sequencing Biology Virology [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] DNA sequencing Aquatic organisms 03 medical and health sciences Aquatic virology 030104 developmental biology Infectious Diseases Fish Viruses [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Next-generation sequencing Nucleic acid sequencing Fish Diagnostics |
| Zdroj: | Intervirology Intervirology, Karger, 2016, 59 (5-6), pp.285-300. ⟨10.1159/000477808⟩ Intervirology, 2016, 59 (5-6), pp.285-300. ⟨10.1159/000477808⟩ |
| ISSN: | 0300-5526 1423-0100 |
| DOI: | 10.1159/000477808⟩ |
| Popis: | International audience; The recent technological advances in nucleic acid sequencing, called next-generation sequencing (NGS), have revolutionized the field of genomics and have also influenced viral research. Aquatic viruses, and especially those infecting fish, have also greatly benefited from NGS technologies, which provide a huge amount of molecular information at a low cost in a relatively short period of time. Here, we review the use of the current high-throughput sequencing platforms with a special focus on the associated challenges (regarding sample preparation and bioinformatics) in their applications to the field of aquatic virology, especially for: (i) discovering novel viruses that may be associated with fish mortalities, (ii) elucidating the mechanisms of pathogenesis, and finally (iii) studying the molecular epidemiology of these pathogens. |
| Databáze: | OpenAIRE |
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