Simultaneous determination of tryptophan, 5-hydroxytryptophan, 5-hydroxytryptamine, 5-hydroxyindoleacetic acid, 4-hydroxy-3-methoxyphenylacetic acid and 3-methoxy-4-hydroxyphenylglycol in human cerebrospinal fluid
| Autor: | Yong-Hong Yi, Xuefen Lu, Wei-Ping Liao |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Male
Serotonin High-performance liquid chromatography Methoxyhydroxyphenylglycol 5-Hydroxytryptophan chemistry.chemical_compound medicine Humans Child Chromatography High Pressure Liquid Chromatography 5-Hydroxyindoleacetic acid Homovanillic acid Tryptophan Temperature Infant Homovanillic Acid General Chemistry Metabolism Hydrogen-Ion Concentration Hydroxyindoleacetic Acid chemistry Child Preschool Tuberculosis Meningeal 3-Methoxy-4-hydroxyphenylglycol Female medicine.drug |
| Zdroj: | Journal of chromatography. B, Biomedical applications. 661(1) |
| ISSN: | 1572-6495 |
| Popis: | Serotonin (5-hydroxytryptamine) metabolism may be influenced by its precursor tryptophan. A method utilizing reversed-phase high-performance liquid chromatography and electrochemical and ultraviolet detection with a mobile phase composed of acetate buffer and methanol has been developed for determination of tryptophan, its metabolites 5-hydroxytryptophan, serotonin, 5-hydroxyindoleacetic acid, as well as 4-hydroxy-3-methoxyphenylacetic acid (homovanillic acid) and 3-methoxy-4-hydroxyphenylglycol in human cerebrospinal fluid (CSF). The electrochemical potential is set at 0.6 V in order to reduce the background current. Since tryptophan is not electroactive at this potential, it is detected by ultraviolet absorbance. The present method is simple, rapid, specific and accurate as compared with a previously reported method. No sample pretreatment is necessary and it takes ca. 20 min to run a sample. The concentrations of the compounds measured in CSF are similar to those obtained by HPLC in previous reports, although there are still arguments about the true level of serotonin in CSF. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|