Bismuth ions inhibit the biological activity of non-amidated gastrins in vivo
| Autor: | Graham S. Baldwin, Suzana Kovac, Su-Wen Loh, Arthur Shulkes, Shamilah Lachal |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
inorganic chemicals Colon Iron medicine.medical_treatment Mice Transgenic Peptide hormone Biochemistry Article Rats Sprague-Dawley Mice In vivo Gastrins Organometallic Compounds medicine Animals Gastrin Pharmacology Cell growth Chemistry Growth factor Biological activity Molecular biology In vitro Rats Ki-67 Antigen Ferric Female Protein Binding medicine.drug |
| Zdroj: | Biochemical Pharmacology. 83:524-530 |
| ISSN: | 0006-2952 |
| Popis: | The peptide hormone gastrin binds two ferric ions with high affinity, and iron binding is essential for the biological activity of non-amidated gastrins in vitro and in vivo. Bi3+ ions also bind to glycine-extended gastrin17 (Ggly), but inhibit Ggly-induced cell proliferation and migration in gastrointestinal cell lines in vitro. The aims of the present study were firstly, to establish the mechanism by which Bi3+ ions inhibit the binding of Fe3+ ions to Ggly, and secondly, to test the effect of Bi3+ ions on the activity of non-amidated gastrins in vivo. The interaction between Bi3+ ions, Fe3+ ions and Ggly was investigated by ultraviolet spectroscopy. The effect of Bi3+ ions on colorectal mucosal proliferation was measured in three animal models. In vitro in the presence of Bi3+ ions the affinity of Fe3+ ions for Ggly was substantially reduced; the data was better fitted by a mixed, rather than a competitive, inhibition model. In rats treated with Ggly alone proliferation in the rectal mucosa was increased by 318%, but was reduced to control values (p < 0.001) in animals receiving oral bismuth plus Ggly. Proliferation in the colonic mucosa of mice overexpressing Ggly or progastrin was significantly greater than in wild-type mice, but was no greater than control (p < 0.01) in animals receiving oral bismuth. Thus a reduction in the binding of Fe3+ ions to Ggly and progastrin in the presence of Bi3+ ions is a likely explanation for the ability of oral bismuth to block the biological activity of non-amidated gastrins in vivo. |
| Databáze: | OpenAIRE |
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