Molecular Identification of Cryptosporidium Species from Pet Snakes in Thailand
| Autor: | Nongnuch Pinyopanuwat, Jumnongjit Phasuk, Tawin Inpankaew, Wissanuwat Chimnoi, Benjarat Yimming, Ketsarin Kamyingkird, Pornchai Sanyathitiseree, Khampee Pattanatanang |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
animal diseases Rainbow boa Zoology Cryptosporidium complex mixtures DNA Ribosomal Polymerase Chain Reaction 03 medical and health sciences Feces parasitic diseases RNA Ribosomal 18S Animals Cluster Analysis Cryptosporidium serpentis Cryptosporidium muris Morelia spilota Elaphe Phylogeny Cryptosporidium parvum Microscopy snake biology integumentary system Ecology Snakes Pets Sequence Analysis DNA 030108 mycology & parasitology DNA Protozoan biology.organism_classification Lampropeltis triangulum Thailand nested PCR Infectious Diseases Parasitology Boa constrictor Original Article SSU rRNA Polymorphism Restriction Fragment Length |
| Zdroj: | The Korean Journal of Parasitology |
| ISSN: | 1738-0006 |
| Popis: | Cryptosporidium is an important pathogen causing gastrointestinal disease in snakes and is distributed worldwide. The main objectives of this study were to detect and identify Cryptosporidium species in captive snakes from exotic pet shops and snake farms in Thailand. In total, 165 fecal samples were examined from 8 snake species, boa constrictor (Boa constrictor constrictor), corn snake (Elaphe guttata), ball python (Python regius), milk snake (Lampropeltis triangulum), king snake (Lampropeltis getula), rock python (Python sebae), rainbow boa (Epicrates cenchria), and carpet python (Morelia spilota). Cryptosporidium oocysts were examined using the dimethyl sulfoxide (DMSO)-modified acid-fast staining and a molecular method based on nested-PCR, PCR-RFLP analysis, and sequencing amplification of the SSU rRNA gene. DMSO-modified acid-fast staining revealed the presence of Cryptosporidium oocysts in 12 out of 165 (7.3%) samples, whereas PCR produced positive results in 40 (24.2%) samples. Molecular characterization indicated the presence of Cryptosporidium parvum (mouse genotype) as the most common species in 24 samples (60%) from 5 species of snake followed by Cryptosporidium serpentis in 9 samples (22.5%) from 2 species of snake and Cryptosporidium muris in 3 samples (7.5%) from P. regius. |
| Databáze: | OpenAIRE |
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