Optimized immunohistochemistry in combination with image analysis: A reliable alternative to quantitative ELISA determination of uPA and PAI-1 for routine risk group discrimination in breast cancer
| Autor: | O. Behrens, W. Schumm, D. S. Lang, U. Heilenkötter, R Simon, T. Goldmann, E. Vollmer |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Concordance Breast Neoplasms Enzyme-Linked Immunosorbent Assay Risk Assessment chemistry.chemical_compound Risk groups Breast cancer Plasminogen Activator Inhibitor 1 Biomarkers Tumor Humans Medicine Clinical significance Aged Aged 80 and over Tissue microarray business.industry Carcinoma Ductal Breast General Medicine Middle Aged Image Enhancement medicine.disease Immunohistochemistry Urokinase-Type Plasminogen Activator Carcinoma Lobular chemistry Tissue Array Analysis Plasminogen activator inhibitor-1 Female Surgery business Plasminogen activator |
| Zdroj: | The Breast. 22:736-743 |
| ISSN: | 0960-9776 |
| DOI: | 10.1016/j.breast.2012.12.011 |
| Popis: | The determination of the invasion markers urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) has further improved the possibilities for individualized therapy of breast cancer. To date, quantitative measurement by ELISA, that needs large amounts of fresh, frozen material, is the only standardized procedure for diagnostic purposes. Therefore, the aim of this study was the establishment of a reliable alternative method based on immunohistochemistry (IHC) and image analysis requiring only small amounts of fixed tumor tissue. Protein expression of uPA and PAI-1 was analyzed in HOPE-fixed tumor samples using tissue microarrays (TMAs) and semiquantitative image analysis. The results of both methods were significantly correlated and risk assessment showed an overall concordance of 78% (83/107; high- and low-risk) and of 94% (74/79) regarding only high-risk patients. The data demonstrate that optimized IHC in combination with image analysis can provide adequate clinical significance compared to ELISA-derived determination of uPA and PAI-1. |
| Databáze: | OpenAIRE |
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