Reversible Binding of Metal Ions onto Bacterial Layers Revealed by Protonation-Induced ATR-FTIR Difference Spectroscopy
| Autor: | Livia Giotta, Krisztina Nagy, Massimo Trotta, Disma Mastrogiacomo, Ludovico Valli, Francesca Italiano, Francesco Milano, Angela Agostiano |
|---|---|
| Přispěvatelé: | Giotta, Livia, Disma, Mastrogiacomo, Francesca, Italiano, Francesco, Milano, Angela, Agostiano, Krisztina, Nagy, Valli, Ludovico, Massimo, Trotta, Mastrogiacomo, Disma |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Metal ions in aqueous solution
Analytical chemistry Infrared spectroscopy Metal ion binding Protonation Rhodobacter sphaeroides Photochemistry bacterial layer Deprotonation Nickel perfusion-induced difference spectroscopy Ni-carboxylato complexes Spectroscopy Fourier Transform Infrared Electrochemistry General Materials Science Fourier transform infrared spectroscopy Spectroscopy Ions biology Chemistry ATR-FTIR difference spectroscopy Rhodobacter sphaeroide Surfaces and Interfaces Hydrogen-Ion Concentration Condensed Matter Physics biology.organism_classification Heavy metal Attenuated total reflection heavy metal biosorption ATR-FTIR protonable groups on bacterial surface |
| Zdroj: | Langmuir 27 (2011): 3762–3773. doi:10.1021/la104868m info:cnr-pdr/source/autori:Livia Giotta, Disma Mastrogiacomo, Francesca Italiano, Francesco Milano, Angela Agostiano, Krisztina Nagy, Ludovico Valli, and Massimo Trotta/titolo:Reversible Binding of Metal Ions onto Bacterial Layers Revealed by Protonation-Induced ATR-FTIR Difference Spectroscopy/doi:10.1021%2Fla104868m/rivista:Langmuir/anno:2011/pagina_da:3762/pagina_a:3773/intervallo_pagine:3762–3773/volume:27 |
| DOI: | 10.1021/la104868m |
| Popis: | The ability of microorganisms to adhere to abiotic surfaces and the potentialities of attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy have been exploited in order to study protonation and heavy metal binding events onto bacterial surfaces. This work represents the first attempt to apply on bacteria the recently developed method known as perfusion-induced ATR-FTIR difference spectroscopy. Such technique allows measurement of even slight changes in the infrared spectrum of the sample, deposited as a thin layer on an ATR crystal, while an aqueous solution is perfused over its surface. Solutions at different pH have been used for inducing protonation/deprotonation of functional groups lying on the surface of Rhodobacter sphaeroides cells, chosen as a model system. The interaction of Ni2+ with surface protonable groups of this microorganism has been investigated with a double-difference approach exploiting competition between nickel cations and protons. Protonation-induced difference spectra of simple model compounds have been acquired in order to guide band assignment in bacterial spectra, thus allowing identification of major components involved in proton uptake and metal binding. The data collected reveal that carboxylate moieties on the bacterial surface of R. sphaeroides play a role in extracellular biosorption of Ni2+, establishing with this ion relatively weak coordinative bonds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|