A sensitive immunoassay based on fluorescence resonance energy transfer from up-converting nanoparticles and graphene oxide for one-step detection of imidacloprid
| Autor: | Fangfang Si, Tianyi Zhang, Jin-Hao Zhao, Wenlong Liang, Yirong Guo, Rubing Zou, Yunyun Chang, Xvsheng Qiao |
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| Rok vydání: | 2020 |
| Předmět: |
Nanoparticle
01 natural sciences High-performance liquid chromatography Analytical Chemistry law.invention chemistry.chemical_compound Neonicotinoids 0404 agricultural biotechnology Imidacloprid law Limit of Detection Tandem Mass Spectrometry medicine Fluorescence Resonance Energy Transfer Immunoassay Chromatography medicine.diagnostic_test Graphene 010401 analytical chemistry Clothianidin Reproducibility of Results 04 agricultural and veterinary sciences General Medicine Thiacloprid Nitro Compounds 040401 food science 0104 chemical sciences Thiazoles Förster resonance energy transfer chemistry Nanoparticles Graphite Food Science Chromatography Liquid |
| Zdroj: | Food chemistry. 335 |
| ISSN: | 1873-7072 |
| Popis: | In this study, a fluorescence resonance energy transfer (FRET) immunoassay based on graphene oxide (GO) and up-converting nanoparticles (UCNPs) was established for rapid detection of imidacloprid, a commonly-used insecticide. Under 980 nm near-infrared light excitation, emission of UCNPs at 542 nm can be absorbed by the energy acceptor GO. The carboxyl-functionalized GO and UCNPs were coupled with competitive antigen and antibody against imidacloprid. After optimization, the FRET immunoassay showed a wide detection range of 0.08–50 ng/mL to imidacloprid, with cross-reaction toward other three neonicotinoids including imidaclothiz (74.4%), thiacloprid (36.9%) and clothianidin (31.9%). The average recoveries of spiked water, Chinese cabbage, cucumber, honey and tea samples were 76.8%-101.8%. The accuracy and reliability of the FRET immunoassay were verified by UPLC-MS/MS with a good correlation (R2 = 0.9816). In a summary, this study provides a sensitive and one-step method for monitoring imidacloprid residue in food and environmental samples within 1 h. |
| Databáze: | OpenAIRE |
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